Effect of 103Pd Radioactive Stent on Human Cholangiocarcinoma Cell Apoptosis and Expression of Fas Gene_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHAO Xiaodan ¹ , HE Guijin ²

1.Department of General Surgery of Donggang Central Hospital, Donggang 118300, Liaoning Province, China;;
2.Department of Hepatobiliary Surgery and Breast Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China;

Corresponding author：

Keywords：

Human cholangiocarcinoma; 103Pd radioactive stent; Fas gene; Cell apoptosis; TUNEL method; Immunohistochemistry

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Objective To research the effect of γ-ray released from 103Pd radioactive stent on the expression of Fas gene and its relation with apoptosis of cholangiocarcinoma cell and the significances through the establishment of human cholangiocarcinoma model.
Methods The model of nude mouse with implanted human cholangiocarcinoma was established. The mice were divided into study group and control group, 37 MBq 103Pd biliary stent was implanted in the study group and the ordinary metal biliary stent was implanted in the control group. The volume of tumor was measured, the cell apoptosis was detected by the TUNEL method and the expression of Fas gene of the cell apoptosis of the induced human cholangiocarcinoma was checked out by immunohistochemistry staining 10 d after the implantation.
Results Compared with the control group, the growing speed of the volume of tumor in study group was significantly reduced (P lt;0.05), the expression positive rate of Fas gene was significantly higher (P lt;0.05), and the apoptotic rate of cancer cells was also higher (P lt;0.01).
Conclusions The 103Pd radioactive stent can induce the cell apoptosis in nude mouse model with implanted human cholangiocarcinoma inhibit the cell growth of bile duct cancer and may promote the apoptosis of cancer cells by increasing the expression of Fas gene. It may be helpful for the further study of treatment for bile duct cancer using 103Pd radioactive stent.

Citation： ZHAO Xiaodan ,HE Guijin. Effect of 103Pd Radioactive Stent on Human Cholangiocarcinoma Cell Apoptosis and Expression of Fas Gene. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(3): 224-228. doi: Copy

1.	Itamochi H, Yamasaki F, Sudo T, et al. Reduction of radiationinduced apoptosis by specific expression of Bcl2 in normal cells [J]. Cancer Gene Ther, 2006; 13(5): 451459.
2.	Peschel RE, Colberg JW, Chen Z, et al. Iodine 125 versus palladium 103 implants for prostate cancer: clinical outcomes and complications [J]. Cancer J, 2004; 10(3): 170174.
3.	Waksman R, Raizner AE, Yeung AC, et al. Use of localised intracoronary beta radiation in treatment of in stent restenosis: the INHIBIT randomised controlled trial [J]. Lancet, 2002; 359(9306): 551557.
4.	何贵金, 于发强, 高沁怡, 等. 103钯放射性支架对Fas基因表达影响及与胆管癌细胞凋亡关系研究 [J]. 中国普外基础与临床杂志, 2007; 14(3): 284286.
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6.	Patel T. Cholangiocarcinoma [J]. Nat Clin Pract Gastroenterol Hepatol, 2006; 3(1): 3342.
7.	Nakeeb A, Pitt HA, Sohn TA, et al. Cholangiocarcinoma. A spectrum of intrahepatic, perihilar, and distal tumors [J]. Ann Surg, 1996; 224(4): 463473.
8.	Ahrendt SA, Pitt HA. Biliary tract [A]. In: Townsend. Sabiston Textbook of Surgery [M]. 16th ed. Philadelphia: WB Saunders Company, 2003: 10761111.
9.	Catelas I, Petit A, Zukor DJ, et al. Cytotoxic and apoptotic effects of cobalt and chromium ions on J774 macrophages-Implication of caspase3 in the apoptotic pathway [J]. J Mater Sci Mater Med, 2001; 12(1012): 949953.
10.	Uno M, Otsuki T, Yata K, et al. Participation of Fasmediated apoptotic pathway in KB, a human head and neck squamous cell carcinoma cell line, after irradiation [J]. Int J Oncol, 2002; 20(3): 617622.
11.	Chakraborty M, Abrams SI, Coleman CN, et al. External beam radiation of tumors alters phenotype of tumor cells to render them susceptible to vaccinemediated Tcell killing [J]. Cancer Res, 2004; 64(12): 43284337.
12.	Yamamoto T, Yoneda K, Ueta E, et al. Enhanced apoptosis of squamous cell carcinoma cells by interleukin2activated cytotoxic lymphocytes combined with radiation and anticancer drugs [J]. Eur J Cancer, 2000; 36(15): 20072017.
13.	Mundle SD, Raza A. Defining the dynamics of selfassembled Fasreceptor activation [J]. Trends Immunol， 2002; 23(4): 187194.
14.	Hengartner MO. The biochemistry of apoptosis [J]. Nature, 2000; 407(6805): 770776.
15.	Kaufmann SH, Earnshaw WC. Induction of apoptosis by cancer chemotherapy [J]. Exp Cell Res, 2000; 256(1): 4249.
16.	Khosravi R, Maya R, Gottlieb T, et al. Rapid ATMdependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage [J]. Proc Natl Acad Sci USA, 1999; 96(26): 1497314977.
17.	Schuler M, Green DR. Mechanisms of p53dependent apoptosis [J]. Biochem Soc Trans, 2001; 29(Pt 6): 684688.
18.	Sheard MA. Ionizing radiation as a responseenhancing agent for CD95mediated apoptosis [J]. Int J Cancer, 2001; 96(4): 213220.
19.	Bratton SB, MacFarlane M, Cain K, et al. Protein complexes activate distinct caspase cascades in death receptor and stressinduced apoptosis [J]. Exp Cell Res, 2000; 256(1): 2733.

1. Itamochi H, Yamasaki F, Sudo T, et al. Reduction of radiationinduced apoptosis by specific expression of Bcl2 in normal cells [J]. Cancer Gene Ther, 2006; 13(5): 451459.
2. Peschel RE, Colberg JW, Chen Z, et al. Iodine 125 versus palladium 103 implants for prostate cancer: clinical outcomes and complications [J]. Cancer J, 2004; 10(3): 170174.
3. Waksman R, Raizner AE, Yeung AC, et al. Use of localised intracoronary beta radiation in treatment of in stent restenosis: the INHIBIT randomised controlled trial [J]. Lancet, 2002; 359(9306): 551557.
4. 何贵金, 于发强, 高沁怡, 等. 103钯放射性支架对Fas基因表达影响及与胆管癌细胞凋亡关系研究 [J]. 中国普外基础与临床杂志, 2007; 14(3): 284286.
5. He GJ, Sun DD, Ji DW, et al. Induction of biliary cholangiocarcinoma cell apoptosis by 103Pd cholangial radioactive stent γrays [J]. Chin Med J (Engl), 2008; 121(11): 10201024.
6. Patel T. Cholangiocarcinoma [J]. Nat Clin Pract Gastroenterol Hepatol, 2006; 3(1): 3342.
7. Nakeeb A, Pitt HA, Sohn TA, et al. Cholangiocarcinoma. A spectrum of intrahepatic, perihilar, and distal tumors [J]. Ann Surg, 1996; 224(4): 463473.
8. Ahrendt SA, Pitt HA. Biliary tract [A]. In: Townsend. Sabiston Textbook of Surgery [M]. 16th ed. Philadelphia: WB Saunders Company, 2003: 10761111.
9. Catelas I, Petit A, Zukor DJ, et al. Cytotoxic and apoptotic effects of cobalt and chromium ions on J774 macrophages-Implication of caspase3 in the apoptotic pathway [J]. J Mater Sci Mater Med, 2001; 12(1012): 949953.
10. Uno M, Otsuki T, Yata K, et al. Participation of Fasmediated apoptotic pathway in KB, a human head and neck squamous cell carcinoma cell line, after irradiation [J]. Int J Oncol, 2002; 20(3): 617622.
11. Chakraborty M, Abrams SI, Coleman CN, et al. External beam radiation of tumors alters phenotype of tumor cells to render them susceptible to vaccinemediated Tcell killing [J]. Cancer Res, 2004; 64(12): 43284337.
12. Yamamoto T, Yoneda K, Ueta E, et al. Enhanced apoptosis of squamous cell carcinoma cells by interleukin2activated cytotoxic lymphocytes combined with radiation and anticancer drugs [J]. Eur J Cancer, 2000; 36(15): 20072017.
13. Mundle SD, Raza A. Defining the dynamics of selfassembled Fasreceptor activation [J]. Trends Immunol， 2002; 23(4): 187194.
14. Hengartner MO. The biochemistry of apoptosis [J]. Nature, 2000; 407(6805): 770776.
15. Kaufmann SH, Earnshaw WC. Induction of apoptosis by cancer chemotherapy [J]. Exp Cell Res, 2000; 256(1): 4249.
16. Khosravi R, Maya R, Gottlieb T, et al. Rapid ATMdependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage [J]. Proc Natl Acad Sci USA, 1999; 96(26): 1497314977.
17. Schuler M, Green DR. Mechanisms of p53dependent apoptosis [J]. Biochem Soc Trans, 2001; 29(Pt 6): 684688.
18. Sheard MA. Ionizing radiation as a responseenhancing agent for CD95mediated apoptosis [J]. Int J Cancer, 2001; 96(4): 213220.
19. Bratton SB, MacFarlane M, Cain K, et al. Protein complexes activate distinct caspase cascades in death receptor and stressinduced apoptosis [J]. Exp Cell Res, 2000; 256(1): 2733.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of 103Pd Radioactive Stent on Human Cholangiocarcinoma Cell Apoptosis and Expression of Fas Gene

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