Objective To observe the effects of vascular endothelial growth factor antisense oligonucleotide (VEGF-ASODN) on expression of vascular endothelial growth factor (VEGF) and growth in gastric cancer cells. Methods The VEGF-ASODN was synthesized artificially with phosphorothioic acid. After transfecting with VEGF-ASODN in gastric cancer cells SGC-7901, the initial copy number of mRNA was detected by real-time RT-PCR, and the quantity of VEGF protein in both cell and supernatant were detected by ELISA. The levels of expression of survivin protein in cells were measured by Western blot. FCM and MTT method were used to detect cellular apoptosis and the activity of cells, respectively. The effect of transfection on the growth of cells was evaluated by growth curve. Results The copy number of VEGR mRNA, protein levels of VEGF in the cells and in culture fluid all decreased when the concentration of transfected VEGF-ASODN increased, as well as the levels of survivin protein （P＜0.05）. The ratio of apoptosis increased, the activity of cells also decreased as the concentration of transfected VEGF-ASODN increased （P＜0.05）. Conclusion Transfection with VEGF-ASODN in gastric cancer cells SGC-7901 can inhibit the expressions of VEGF and survivin remarkably. It can enhance cellular apoptosis and suppress growth of cells.
Citation：
ZHENG Bin,DUAN Tide,ZUO Tie. Suppression of VEGF-ASODN on Expression of VEGF and Growth in Gastric Cancer Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(5): 347-351. doi:
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- 1. Hashimoto M, Ohsawa M, Ohnishi A, et al. Expression of vascular endothelial growth factor and its receptor mRNA in angiosarcoma [Ｊ]. Lab Invest, 1995; 73(6)∶859.
- 2. Plate KH. Control of tumor growth via inhibition of tumor angiogenesis [Ｊ]. Adv Exp Med Biol, 1998; 451∶57.
- 3. Chan AS, Leung SY, Wong MP, et al. Expression of vascular endothelial growth factor and its receptors in the anaplastic progression of astrocytoma, oligodendroglioma, and ependymoma [Ｊ]. Am J Surg Pathol, 1998; 22(7)∶816.
- 4. 敬静， 吕青， 李宏江, 等. 乳腺癌患者血清和肿瘤组织VEGF表达与临床预后的关系 [Ｊ]. 中国普外基础与临床杂志，2006; 13(6)∶705.
- 5. 刘智敏， 严律南， 赵永恒, 等. 胆管癌组织中VEGF和c-myc的表达及其意义 [Ｊ]. 中国普外基础与临床杂志， 2004; 11(2)∶139.
- 6. 梁燕， 姜军， 王清良， 等. 自制组织芯片研究VEGF-C在乳腺癌中的表达及其意义 [Ｊ]. 中国普外基础与临床杂志， 2006; 13(5)∶580.
- 7. 解乃昌， 李国威， 王志亮, 等. 抗血管内皮生长因子抗体对实验性肝癌的生长抑制作用 [Ｊ]. 中国普外基础与临床杂志， 2002; 9(4)∶242.
- 8. Buolamwini JK. Novel anticancer drug discovery [Ｊ]. Curr Op-in Chem Biol, 1999; 3(4)∶500.
- 9. 陈曦海， 张岂凡， 马荣. 反义survivin诱导胃癌SGC-7901细胞凋亡及逆转耐药的研究 [Ｊ]. 中国普外基础与临床杂志, 2007; 14（1）∶63.
- 10. Mesri M, Maneul MR, Elizabeth J, et al. Suppression of vascular endothelial growth mediated endothelial cell protection by surviving targeting [Ｊ]. Am J Pathol, 2001; 158（5）∶1757.
- 11. 杨军， 胡新华， 刘程伟， 等. 反义寡脱氧核苷酸抑制survivin基因表达对移植静脉内膜增生的影响 [Ｊ]. 中国普外基础与临床杂志, 2006; 13（6）∶647.
- 12. Li F, Ambrosini G, Chu EY, et al. Control of apoptosis and mitotic spindle check point by survivin [Ｊ]. Nature (Lond), 1998; 396(6711)∶580.
- 13. Hengartner MO. The biochemistry of apoptosis [Ｊ]. Nature, 2000; 407(6805)∶770.
