Effects of As2O3 on Expression of NF-κB p65, Survivin and Caspase-3 in Human Breast Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

MA Yinbin , WU Chengyi , CHEN Xin.

Department of General Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China;

Corresponding author：

Keywords：

Arsenic trioxide Breast neoplasm Gene

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【Abstract】ObjectiveTo investigate the effects of As2O3 on expression of NF-κB p65, survivin and caspase-3 in human breast infiltrating duct carcinoma xenograft model on nude mice.
Methods A human breast infiltrating duct carcinoma model on nude mice was established and the nude mice were divided randomly into three groups: control group, DDP group and As2O3 group （1.5 and 3.0 mg/kg concentrations）. The expression of survivin mRNA was detected with the method of in situ hybridization and the expressions of NF-κB p65, survivin and caspase-3 protein were measured with immunohistochemistry.
ResultsThe positive rates of NF-κB p65 and survivin expression were higher in the control group than those in the DDP group and the As2O3 groups, but that of caspase-3 was on the opposite way (P＜0.01). The positive rates of NF-κB p65 and survivin in As2O3 group were negatively related with the concentrations of As2O3 (P＜0.01), but that of caspase-3 was on the opposite way (P＜0.01). The expressions of NF-κB p65 and survivin protein were positively correlated with that of survivin mRNA, but any of them was negatively correlated with the expression of caspase-3 protein.
ConclusionAs2O3 inhibites survivin probably by inhibiting the activity of NFκB p65 and subsequently activates caspase-3, which induces apoptosis of human breast infiltrating duct carcinoma cells and is in a dose-dependent manner.

Citation： MA Yinbin,WU Chengyi,CHEN Xin.. Effects of As2O3 on Expression of NF-κB p65, Survivin and Caspase-3 in Human Breast Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(4): 409-412. doi: Copy

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2. 陈鑫，吴诚义. 三氧化二砷对人乳腺癌裸鼠移植瘤的作用 [Ｊ]. 中华实验外科杂志， 2003; 20(8)∶722.
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4. Nakshatri H, Goulet RJ Jr. NFkappaB and breast cancer [Ｊ].
5. Takada Y, Khuri FR, Aggarwal BB. Protein farnesyltransferase inhibitor (SCH 66336) abolishes NFkappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NFkappa Bregulated gene expression and upregulation of apoptosis [Ｊ]. J Biol Chem， 2004; 279(25)∶26287.
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8. Bharti AC, Aggarwal BB. Chemopreventive agents induce suppression of nuclear factorkappaB leading to chemosensitization [Ｊ]. Ann NY Acad Sci， 2002; 973∶392.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of As2O3 on Expression of NF-κB p65, Survivin and Caspase-3 in Human Breast Cancer

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