A Comparative Study on Sensitivity of HE,IHC and RTPCR in Detection of Breast Cancer Metastases in Axillary Lymph Nodes_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Bo ¹ , LU Yunfei ² , ZENG Jian ² , HE Sheng. ¹

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Keywords：

Breast cancer Axillary lymph nodes Immunohistochemistry RT-PCR Metastasis

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【Abstract】Objective To compare the sensitivity of HE,immunohistochemistry (IHC) and RT-PCR in detection of breast cancer metastases in axillary lymph nodes.
MethodsTwenty female patients with newly diagnosed and clinically nodenegative breast cancers underwent modified radical mastectomy, including a complete axillary lymph node dissection. The ages of the patients ranged from 31 years to 65 years, and the diagnosis of breast cancer was approved by pathological finding. Two hundred and thirty-nine axillary lymph nodes were found in these 20 patients. Metastases in axillary lymph nodes were explored by HE， cytokeratin 19 IHC and RT-PCR for cytokeratin 19 respectively.
ResultsSeven(2.9%) lymph nodes were found to have metastatic cancers by HE in 3 patients,all nodes were found in level Ⅰ. Metastatic cancers were found in 13(5.4%) nodes by IHC in 7 patients,11 nodes in level Ⅰ and 2 nodes in level Ⅱ; and 52(21.8%) nodes were found to contain tumor cells by RT-PCR in 14 patients,30 nodes in level Ⅰ and 22 nodes in level Ⅱ. All of 7 histologically(HE) positive nodes were found to contain tumor cells by IHC and RT-PCR. Among 232 histologically(HE) negative nodes,6(2.6%) nodes were found to contain tumor cells by IHC,and 45(19.4%) nodes were found to contain tumor cells by RT-PCR, all 6 IHC positive nodes showed the expected 460-base pair products on gel electrophoresis (P＜0.05).
ConclusionThis study suggests that IHC and RT-PCR are more sensitive methods for the detection of micrometastases of breast cancer in lymph nodes than HE is,and RT-PCR is even better than IHC; the micrometastases of breast cancer in axillary lymph nodes could be detected accurately through these techniques.

Citation： CHEN Bo,LU Yunfei,ZENG Jian,HE Sheng.. A Comparative Study on Sensitivity of HE,IHC and RTPCR in Detection of Breast Cancer Metastases in Axillary Lymph Nodes. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(3): 238-240. doi: Copy

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1. Traweek ST, Liu J, Battifora H. Keratin gene expression in nonepithelial tissues. Detection with polymerase chain reaction [Ｊ]. Am J Pathol， 1993; 142(4)∶1111.
2. Noguchi S, Aihara T, Motomura K, et al. Detection of breast cancer micrometastases in axillary lymph nodes by means of reverse transcriptasepolymerase chain reaction. Comparison between MUC1 mRNA and keratin 19 mRNA amplification [Ｊ]. Am J Pathol, 1996; 148(2)∶649.
3. Schoenfeld A, Luqmani Y, Smith D, et al. Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction [Ｊ]. Cancer Res, 1994; 54(11)∶2986.
4. International(Ludwig)Breast Cancer Study Group.Prognostic importance of occult axillary lymph node micrometastases from breast cancer [Ｊ]. Lancet, 1990; 335(8705)∶1565.
5. Noguchi S, Aihara T, Nakamori S, et al. The detection of breast carcinoma micrometastases in axillary lymph nodes by means of reverse transcriptasepolymerase chain reaction [Ｊ]. Cancer, 1994; 74(5)∶1595.
6. Silverstein MJ, Gierson ED, Waisman JR, et al. Axillary lymph node dissection for T1a breast carcinoma. Is it indicated? [Ｊ]. Cancer, 1994; 73(3)∶664.
7. Giuliano AE, Kirgan DM, Guenther JM, et al. Lymphatic mapping and sentinel lymphadenectomy for breast cancer [Ｊ]. Ann Surg, 1994; 220(3)∶391.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

A Comparative Study on Sensitivity of HE,IHC and RTPCR in Detection of Breast Cancer Metastases in Axillary Lymph Nodes

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