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【Abstract】ObjectiveTo develop a new singletube polymerase chain reaction amplification (ST Amp) protocol for the efficient sequencing-based typing (SBT) of human leukocyte antigen DRB1(HLA-DRB1).
MethodsA set of 7 group-specific exonic 5′ amplification primers and a single generic 3′ primer were included together in a single PCR mix to facilitate a single PCR amplification per sample for HLA-DRB1 typing.
ResultsAll samples were successfully typed, the typing result was accurate and repeatable.
ConclusionST Amp technique has resulted in the ability to perform high-resolution, high-specificity and high-throughput HLA-DRB1 typing by DNA sequencing.

Citation: CHEN Gengzhen,ZHOU Zongguang,ZHENG Xuelian,ZHOU Bin,GU Jun.. Single-Tube Polymerase Chain Reaction Amplification Protocol for SequencingBased Typing of Human Leukocyte Antigen DRB1. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(3): 258-260. doi: Copy

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