Detection of Human Papilloma Virus 16 E7 DNA and Protein in Patients with Colorectal Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Jiancheng , ZHOU Zongguang , ZHENG Xuelian , ZHOU Bin , LI Hongguang , GU Jun , WANG Rong.

Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

Colorectal neoplasms Human papilloma virus HPV16 E7 protein

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【Abstract】ObjectiveTo detect the expression of human papilloma virus(HPV) 16 E7 was detected in colorectal adenocarcinoma tissue and normal mucosa.
MethodsEighty-two patients with primary colorectal adenocarcinoma were selected in this study. The samples were taken from the tumor and the adjacent normal mucosa (10 cm away from the tumor) in each patient. Polymerase chain reaction (PCR) and immunohistochemistry were used to detect HPV16 E7 DNA and protein respectively.
ResultsHPV16 E7 DNA expression was significantly higher in colorectal carcinoma (51.22%,42/82) than that in adjacent normal mucosa (4.88%,4/82), P＜0.01. A correlation was found between HPV16 E7 DNA expression and tumor location (P＜0.05)，18.18% in the ascending colon carcinoma and 64.10% in the rectal carcinoma. HPV16 E7 DNA expression was also associated with Dukes stage（P＜0.01）, but was not correlated with cancer differentiation. HPV16 E7 protein expression was mainly dectected in the nuclei of tumor cells with immunohistochemistry. There was a correlation between the expression of HPV16 E7 protein and HPV16 E7 gene. PCR had a higher sensitivity than immunohistochemistry.
ConclusionHPV16 infection rate is much higher in the colorectal carcinoma than that in the adjacent normal mucosa, which indicates that HPV16 infection exists in some colorectal carcinomas. The high infection rate of HPV16 E7 is associated with advanced Dukes stage and proximity to anus.

Citation： ZHANG Jiancheng,ZHOU Zongguang,ZHENG Xuelian,ZHOU Bin,LI Hongguang,GU Jun,WANG Rong.. Detection of Human Papilloma Virus 16 E7 DNA and Protein in Patients with Colorectal Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(3): 280-283下转289. doi: Copy

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1. Lee YM, Leu SY, Chiang H, et al. Human papilloma virus type 18 in colorectal cancer [Ｊ]. J Microbiol Immunol Infect, 2001; 34(2)∶87.
2. McGregor B, Byrne P, Kirgan D, et al. Confirmation of the association of human papilloma virus with human colon cancer [Ｊ]. Am J Surg, 1993; 166(6)∶738.
3. Audeau A, Han HW, Johnston MJ, et al. Does human papilloma virus have a role in squamous cell carcinoma of the colon and upper rectum? [Ｊ]. Eur J Surg Oncol, 2002; 28(6)∶657.
4. Buyru N, Budak M, Yazici H, et al. p53 gene mutations are rare in human papilloma virusassociated colon cancer [Ｊ]. Oncol Rep, 2003; 10(6)∶2089.
5. Bosch FX, Munoz N. The viral etiology of cervical cancer [Ｊ]. Virus Res, 2002; 89(2)∶183.
6. Frisch M, Smith E, Grulich A, et al. Cancer in a populationbased cohort of men and women in registered homosexual part（下转第289页）（上承第283页）nerships [Ｊ]. Am J Epidemiol, 2003; 157(11)∶966.
7. Cheng JY, Sheu LF, Meng CL, et al. Detection of human papilloma virus DNA in colorectal carcinomas by polymerase chain reaction [Ｊ]. Gut, 1995; 37(1)∶87.
8. Kirgan D, Manalo P, McGregor B. Immunohistochemical demonstration of human papilloma virus antigen in human colon neoplasms [Ｊ]. J Surg Res, 1990; 48(5)∶397.
9. Shah KV, Daniel RW, Simons JW, et al. Investigation of colon cancers for human papilloma virus genomic sequences by polymerase chain reaction [Ｊ]. J Surg Oncol, 1992; 51(1)∶5.
10. Cheng JY, Meng CL, Chao CF, et al. Human papilloma virus 16 DNA in NIH3T3 cells transformed by colonic cancer cellular DNA [Ｊ]. Gut, 1993; 34(12)∶1710.
11. Weinberger PM, Yu Z, Zerkowski M, et al. A possible association of human papilloma virus with a subset of colorectal adenocarcinomas [Ｊ]. J Clin Oncol, 2004; 22(14S)∶3544.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Detection of Human Papilloma Virus 16 E7 DNA and Protein in Patients with Colorectal Carcinoma

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