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【Abstract】Objective To study the regulatory ability of peroxisome proliferatoractivated receptor γ(PPARγ) ligands to the inflammatory response in human gallbladder epithelial cells.
Methods Culture human gallbladder epithelial cells and identify them . Cells were treated for 24 hours with 0, 10 μmol/L, 20 μmol/L, 30 μmol/L, 50 μmol/L and 100 μmol/L of Ciglitazone during cellular growth peak(5th day), then stimulated them with hIL-1β 5 ng/ml for 2 hours and measured the concentration of IL-6、IL-8 and TNF-α in cellular supernatants by riadioimmunoassay.
Results Contrasted with control group, the expression of IL-6 and IL-8 in each test group were inhibited (P<0.001). The IL-6 and IL-8 levels were gradually dropped and corelated with the dosage of Cigtitazone, and manifested dosagedependence (P<0.001). The concentration of TNF-α could not be measured.
Conclusion PPARγ ligands can inhibit the expression of IL-6 and IL-8 in human gallbladder epithelial cells and probably produce effect in the regulation of cholecystic inflammation.

Citation: LIU Jiangwen,CHENG Nansheng,PAN Guangdong,XIONG Xianze,YAN Lan.. Experimental Study on PPARγ Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(4): 336-339. doi: Copy

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