Effects of Shear Stress on the Secretions of Sinusoidal Endothelial Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

DING Jiming , CHEN Ping , ZHANG Lin , ZHU Jin , XIONG Yan , DUAN Shigang , LI Ying.

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Keywords：

Shear stress Sinusoidal endothelial cell Secretion function Cytokine

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【Abstract】Objective To observe concentration changes of substances including hepatocyte growth factor(HGF),interleukin-6(IL-6),nitric oxide(NO),nitric oxide synthase(NOS) that were secreted by sinusoidal endothelial cells(SECs) in different shear stress and study the effects of shear stress on the secretions of SECs.
Methods To establish a hemodynamics equipment and to set up a way to separate and culture SEC. The rats were divided into two groups: the control group (shear stress was 0 dyn/cm2) and study group. Study group was divided into three subgroups according to shear stress (12, 24 and 48 dyn/cm2). Sinusoidal endothelial cell culture mediums were examined in different shear stress and phases.
Results HGF, IL-6, NO and NOS secreted by sinusoidal endothelial cells in different shear stress were distinctive and had positive correlation with shear stress and action time.
Conclusion In vitro, the secretion of HGF,IL-6,NO and NOS of sinusoidal endothelial cells are increased along with shear stress rising, which suggests that the rising pressure in portal vein after hepatectomy may result in SECs activated．Activated SECs then secret many cytokines and medias to trigger the regeneration of liver.

Citation： DING Jiming,CHEN Ping,ZHANG Lin,ZHU Jin,XIONG Yan,DUAN Shigang,LI Ying.. Effects of Shear Stress on the Secretions of Sinusoidal Endothelial Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(5): 455-458下转468. doi: Copy

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28.	李黔宁,应大君,戴光明,等. 一种简易的平行板流动腔系统的应用 [Ｊ]. 医用生物力学， 2002； 17（1）∶7.
29.	Frangos JA, Eskin SG, McIntire LV, et al. Flow effects on prostacyclin production by cultured human endothelial cells [Ｊ]. Science, 1985; 227(4693)∶1477.

1. Kahn D, van HoornHickman R, Terblanche J. Liver blood flow after partial hepatectomy in the pig [Ｊ]. J Surg Res, 1984; 37(4)∶290.
2. LeCouter J, Moritz DR, Li B, et al. Angiogenesisindependent endothelial protection of liver: role of VEGFR1 [Ｊ]. Science, 2003; 299(5608)∶890.
3. Xu B, Broome U, Uzunel M, et al. Capillarization of hepatic sinusoid by liver endothelial cellreactive autoantibodies in patients with cirrhosis and chronic hepatitis [Ｊ]. Am J Pathol, 2003; 163(4)∶1275.
4. Mendoza L, Carrascal T, De Luca M, et al. Hydrogen peroxide mediates vascular cell adhesion molecule1 expression from interleukin18activated hepatic sinusoidal endothelium: implications for circulating cancer cell arrest in the murine liver [Ｊ]. Hepatology, 2001; 34(2)∶298.
5. 朱瑾, 陈平,熊燕, 等. 大鼠肝窦内皮细胞分离、培养及其生物学特性的初步研究 [Ｊ]. 消化外科， 2004； 3（5）∶350.
6. Huet PM, Nagaoka MR, Desbiens G, et al. Sinusoidal endothelial cell and hepatocyte death following cold ischemiawarm reperfusion of the rat liver [Ｊ]. Hepatology, 2004; 39(4)∶1110.
7. Nedredal GI, Elvevold KH, Ytrebo LM, et al. Liver sinusoidal endothelial cells represents an important blood clearance system in pigs [Ｊ]. Comp Hepatol, 2003; 2(1)∶1.
8. Limmer A, Knolle PA. Liver sinusoidal endothelial cells: a new type of organresident antigenpresenting cell [Ｊ]. Arch Immunol Ther Exp (Warsz), 2001; 49(Suppl 1)∶S7.
9. Mendoza L, VidalVanaclocha F. Inflammatory response of tumoractivated hepatic sinusoidal endothelium as a target for the screening of metastasis chemopreventive drugs [Ｊ]. Methods Mol Med, 2003; 85∶107.
10. Thomson RY, Clarke AM. Role of portal blood supply in liver regeneration [Ｊ]. Nature, 1965; 208(8)∶392.
11. Schoen JM, Wang HH, Minuk GY, et al. Shear stressinduced nitric oxide release triggers the liver regeneration cascade [Ｊ]. Nitric Oxide, 2001; 5(5)∶453.
12. Kaneko T, Sugimoto H, Inoue S, et al. Doppler ultrasonography for postoperative hepatofugal portal blood flow of the intrahepatic portal vein [Ｊ]. Hepatogastroenterology, 2003; 50(54)∶1825.
13. Sato Y, Tsukada K, Hatakeyama K. Role of shear stress and immune responses in liver regeneration after a partial hepatectomy [Ｊ]. Surg Today, 1999; 29(1)∶1.
14. Eguchi S, Yanaga K, Sugiyama N, et al. Relationship between portal venous flow and liver regeneration in patients after living donor rightlobe liver transplantation [Ｊ]. Liver Transpl, 2003; 9(6)∶547.
15. Hasmall S, James N, Hedley K, et al. Mouse hepatocyte response to peroxisome proliferators: dependency on hepatic nonparenchymal cells and peroxisome proliferator activated receptor alpha (PPARalpha) [Ｊ]. Arch Toxicol, 2001; 75(6)∶357.
16. Malik R, Selden C, Hodgson H. The role of nonparenchymal cells in liver growth [Ｊ]. Semin Cell Dev Biol, 2002; 13(6)∶425.
17. Mochida S, Ishikawa K, Toshima K, et al. The mechanisms of hepatic sinusoidal endothelial cell regeneration: a possible communication system associated with vascular endothelial growth factor in liver cells [Ｊ]. J Gastroenterol Hepatol, 1998; 13(Suppl)∶S1.
18. Sato T, ElAssal ON, Ono T, et al. Sinusoidal endothelial cell（下转第468页）（上承第458页）proliferation and expression of angiopoietin/Tie family in regenerating rat liver [Ｊ]. J Hepatol, 2001; 34(5)∶690.
19. Michalopoulos GK, DeFrances MC. Liver regeneration [Ｊ]. Science, 1997; 276(5309)∶60.
20. Michalopoulos GK. Hepatocyte growth factor in liver growth and differentiation [Ｊ]. J Boil Chem, 1995; 98（11）∶219.
21. Suzuki A, Iwama A, Miyashita H, et al. Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells [Ｊ]. Development, 2003; 130(11)∶2513.
22. Daveau M, Scotte M, Francois A, et al. Hepatocyte growth factor, transforming growth factor alpha, and their receptors as combined markers of prognosis in hepatocellular carcinoma [Ｊ]. Mol Carcinog, 2003; 36(3)∶130.
23. Koniaris LG, McKillop IH, Schwartz SI, et al. Liver regeneration [Ｊ]. J Am Coll Surg, 2003; 197(4)∶634.
24. Debonera F, Aldeguer X, Shen X, et al. Activation of interleukin6/STAT3 and liver regeneration following transplantation [Ｊ]. J Surg Res, 2001; 96(2)∶289.
25. Cressman DE, Greenbaum LE, DeAngelis RA, et al. Liver failure and defective hepatocyte regeneration in interleukin6deficient mice [Ｊ]. Science, 1996; 274(5291)∶1379.
26. Kelm M, Feelisch M, Deussen A, et al. Release of endothelium derived nitric oxide in relation to pressure and flow [Ｊ]. Cardiovasc Res, 1991; 25(10)∶831.
27. Hortelano S, Dewez B, Genaro AM, et al. Nitric oxide is released in regenerating liver after partial hepatectomy [Ｊ]. Hepatology, 1995; 21(3)∶776.
28. 李黔宁,应大君,戴光明,等. 一种简易的平行板流动腔系统的应用 [Ｊ]. 医用生物力学， 2002； 17（1）∶7.
29. Frangos JA, Eskin SG, McIntire LV, et al. Flow effects on prostacyclin production by cultured human endothelial cells [Ｊ]. Science, 1985; 227(4693)∶1477.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Shear Stress on the Secretions of Sinusoidal Endothelial Cells

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