ObjectiveTo explore the MRI manifestations and its diagnostic value for brachial plexus injury. MethodsMRI manifestations and surgery-related materials of 21 patients confirmed to have brachial plexus injury from January 2011 to April 2013 were retrospectively analyzed. ResultsAccording to the classification of brachial plexus injury, preganglionic brachial plexus injury occurred in 13 cases (23 nerves) and postganglionic injury occurred in 8 cases (24 nerves). The manifestations of preganglionic brachial plexus injury in MRI included nerve root disappearance (11) or enlargement (9), nerve root sleeve form abnormality (3), meningeal cyst (9), catheter thickening (6), and spinal cord edema, deformation and displacement (3). Postganglionic injury MRI findings showed nerve trunk enlargement (8), continuity interrupt line (12), rigidity (4), and adjacent structure disorder and edema (6). Surgery confirmed preganglionic brachial plexus injury in 27 nerves and postganglionic injury in 29 nerves. Compared with surgery, the sensitivity, specificity and accuracy of MRI in diagnosing preganglionic brachial plexus injury were 81.5%, 80.0%, and 81.3%, respectively. The sensitivity, specificity and accuracy of MRI in diagnosing postganglionic brachial plexus injury were 79.3%, 85.7%, and 80.6%, respectively. ConclusionsMRI is excellent in revealing preganglionic brachial plexus injury and postganglionic injury. MRI diagnosis accuracy is high, and it is optimal for clinical diagnosis and treatment with no invasion.
ObjectiveTo investigate the value of MRI in the diagnosis of central nervous system lymphoma (CNSL). MethodsWe retrospectively analyzed the clinical data of 20 cases of primary CNSL (PCNSL) and 13 cases of secondary CNSL (SCNSL) from the Second People's Hospital of Chengdu and Chengdu 363 Hospital from January to December 2013, and analyzed their clinical data and MRI image data. We observed the tumor location, tumor size and signal, and carried out the statistical analysis. ResultsTwenty patients had PCNSL in the brain, including single lesion in 9 (45.0%), and multiple in 11 (55.0%). Among the 48 lesions, there were 23 (47.9%) nodular lesions, 21 (43.8%) crumb lesions, and 4 (8.3%) dot patch lesions; MRI showed slightly low T1 signal and slightly high T2 signal in most lesions, and showed significant even enhancing, and mild to moderate edema around the tumor. SCNSL lesions were mainly meningeal disseminated with 3 cases (23.1%) of single lesions and 10 cases (76.9%) of multiple ones, and there were a total of 30 lesions. MRI manifested that T1 and T2 mainly showed equal signals, and showed an obviously even enhancing status, and mild to moderate edema around the tumor. ConclusionThe central nervous system lymphoma has a certain characteristic MRI image, and MRI images of the primary and secondary central nervous system lymphoma were similar.
Objective To investigate the potential mechanism of cellular senescence-related mitochondrial autophagy genes in diabetic retinopathy (DR). MethodsThe DR gene datasets GSE53257 and GSE60436 from the GEO database and screened the differentially expressed genes (DEG) were downloaded; Cellular senescence-related genes (CSRG) and mitochondrial autophagy-related genes from the GeneCards database, and the intersection of the two to obtain the DR-related differentially expressed genes (CSRMRDEG) were collected. The obtained CSRMRDEG was subjected to Gene Ontology (GO) functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis, protein-protein interaction network (PPI) analysis, and hub gene identification using Maximal Clique Centrality (MCC), Degree, Maximum Neighborhood Component (MNC)、Edge Percolated Component (EPC) and Closeness algorithms. Gene Set Enrichment Analysis (GSEA) was conducted to obtain the enriched pathways of DEG, and ssGSEA immune infiltration analysis was performed to screen the correlation between immune cells and DR. The diagnostic efficacy of hub genes for DR was evaluated by drawing the receiver operating characteristic (ROC) curve and calculating the area under the curve (AUC). Meanwhile, the Wilcoxon rank sum test was used to compare the differences in the infiltration level of immune cells between the DR Group and the control group. Results23 DR-related CSRMRDEG were obtained; GO analysis showed that they were mainly enriched in the pathways of dicarboxylic acid, biosynthetic process of folate-containing compounds, tetrahydrofolate conversion, mitochondrial matrix, mitochondrial endomembrane, structural components of ribosomes, and glutamate transmembrane transporter protein activity; The results of KEGG pathway enrichment analysis showed that CSRMRDEG was highly enriched in pathways such as the folate carbon pool, biosynthesis of cofactors, and pyruvate metabolism. The PPI analysis results show that there are 16 related CSRMRDEG. Five algorithms obtained the nine Hub genes. The results of ROC curve analysis showed that the AUC of the expression levels of 9 Hub genes for diagnosing DR Ranged from 0.7-0.9. The ssGSEA results showed that there were statistically significant differences in Wilcoxon of central memory CD4+ T cells, macrophages, natural killer cells, and helper T cell 1 between the DR Group and the control group (Z=−2.85, −2.23, −2.10, −2.52; P＜0.05). ConclusionMitochondrial autophagy genes related to cellular senescence are potential diagnostic targets for DR.