Objective To summarize the recent progress of construction methods of engineered cell sheet and to forecast the possible prospect. Methods The recent original articles about investigation and appl ication of engineered cell sheet were reviewed. Several common methods were selected and expounded. Results The construction methods of engineered cell sheet mainly include temperature-responsive culture dish, salmon atelocollagen, magnetic force, surface roughness, and polyelectrolytes, which may overcome the l imits of traditional tissue engineering methods. Conclusion The construction methods of engineered cell sheet are feasible and have a bright future in the cl inical appl ication.
ObjectiveTo investigate and analyze the ophthalmic resource distribution and service ability of Leshan City, and provide scientific basis for development of ophthalmology and prevention of blindness. MethodsWe statistically analyzed all departments of ophthalmology in 17 general hospitals of Leshan, including numbers of beds, numbers of health technicians, professional title structure, ophthalmic instruments, levels of operation and service ability in 2012. ResultsThere were 186 ophthalmic beds, 84 ophthalmologists, 6 technicians, 64 nurses, 16 professors, 28 doctors with medium-level title, and 40 residents in the 17 general hospitals of Leshan. There were 184 300 out-patients and 9 920 in-patients with 12 320 operations including 6 211 cataract operations in the year of 2012. ConclusionThe ophthalmic resources and service ability are not equally distributed in Leshan. Most resources are distributed in big hospitals of the urban district. Meanwhile, hospitals in remote areas do not have ophthalmologists or ophthalmologic instruments. We should develop our service ability and work efficiency by continuous learning in order to improve the three-level primary blindness prevention system.
Objective We aimed to evaluate the current status of the construction and practice of the stroke center in West China Hospital of Sichuan University and develope a future strategy to promote the standardized developement of inpatients care and evidence practice. Methods The current status of the Stroke Center in West China Hospital of Sichuan University was assessed. The procedure of diagnosis and treatment was inspected in detail, including triage, thrombolytic therapy, antithrombotic medication and anticoagulant, primary and secondary prevention, filter of risk factors, rehabilitation and education for patients. After that, new plans were made on the basis of the assessment and experiences acquired from practices in the Stroke Center in West China Hospital. Results The primary Stroke Center had been constructed in West China Hospital. The practice in West China Hospital showed that the Stroke Center significantly reduced the mortality and shortened the length of hospital stay of the patients with stroke. Conclusion It is proved that construction and implementation of the Stroke Center in West China Hospital are feasible.
Objective To compare postoperative survival rates and the incidence of adverse events in patients with three-vessel disease undergoing complete versus incomplete revascularization during coronary artery bypass grafting (CABG). Methods A retrospective analysis was conducted on patient data from Tianjin Chest Hospital who underwent primary isolated CABG surgery between 2019 and 2020. Patients were divided into a complete revascularization group and an incomplete revascularization group based on the revascularization status after surgery. Inverse probability of treatment weighting (IPTW) was used for risk adjustment. Results A total of 1 419 patients were included in the study, with 1 086 (76.5%) undergoing complete revascularization. IPTW analysis showed that complete revascularization could reduce the incidence of major adverse cardiovascular and cerebrovascular events (MACCE) [HR=0.596, 95%CI (0.404, 0.880), P=0.010] and angina [HR=0.560, 95%CI (0.377, 0.823), P=0.004]. Conclusion In patients with multivessel coronary artery disease, complete revascularization may be associated with improved patient outcomes.
Biobank is an important platform for translational medicine, precision medicine and drug development. In the past 10 years, although the construction of biobank in China has made great progress, it still faces many challenges. This article analyzes the key issues and also puts forward specific suggestions in the management of biobank from the aspects of ethics issues, quality control of biospecimens, information management, sample and data sharing, so as to provide reference for the construction and development of a high standard biobank.
Transcatheter aortic valve replacement (TAVR) has been a crucial treatment for elder patients with aortic stenosis in developed countries, which is still at its beginning in China. TAVR is a risky and complicated technic; and to promise the long-term development of it, we need to build a multiple disciplinary heart team consisted of doctors from different specialties and guided by various disciplines, also to guarantee the team operates well. In order to help Chinese doctors understand heart team well, this article describes component parts and requirement for each member of the team, in aspect of cardiologist, cardiac surgeon, echocardiologist, radiologist, anesthesiologist and nursing team; and discuss team operation mechanism through pre-procedural evaluation, procedural cooperation, peri-procedural management and post-procedural follow-up.
The construction of high-level talent teams is the core of building up high-level universities and hospitals, and it is an important reference index for the ranking of universities and academic disciplines. The first-class medical talent teams is an essential requirement for comprehensive hospitals to be ranked as "Double First-Class". Based on the practice of construction of high-level medical talents in West China Hospital of Sichuan University, this paper introduces the optimal appoaches in this regard.
ObjectiveTo investigate the effectiveness of combined three operations (rotated total upper eyelid skin flap, construction of double eyelid, and "Z" flap epicanthal plasty) for one stage defect repair after resection of xanthelasma palpebrarum with epicanthus. MethodsBetween December 2013 and December 2015, 12 female patients with large xanthelasma palpebrarum and epicanthus underwent rotated total upper eyelid skin flap, construction of double eyelid, and "Z" flap epicanthal plasty for one stage defect repair. The age ranged from 36 to 59 years (mean, 43 years). The course of disease was 3 to 16 years, with an average of 11 years. The initial resection was performed in 6 cases, second resection of residual xanthelasma palpebrarum in 4 cases, and 2 cases had recurrence after resection. The maximum diameter of xanthelasma palpebrarum was 0.5-1.3 cm (mean, 1.0 cm). According to CHE Junmin et al criterion, epicanthus was rated as mild in 7 cases, moderate in 3 cases, and severe in 2 cases. The blood lipid level was in normal range. ResultsPrimary healing of incision was obtained, and the flaps survived in all patients; no complication occurred. Scar hyperplasia was found in 4 cases at 1 month after operation, and the comprehensive treatment of scar was performed. All patients were followed up for 3 months to 2 years, with an average of 1.5 years. Double eyelid effects were good, and no xanthelasma palpebrarum recurred. ConclusionA combination of rotated total upper eyelid skin flap, construction of double eyelid, and "Z" flap epicanthal plasty is an effective operative procedure to repair defect after resection of xanthelasma palpebrarum with epicanthus; and better curve of double eyelid, better shape of endocanthion, and less tension of flap can be got.
Clinical prediction models refer to models that can predict the probability of the occurrence of a certain clinical outcome event of the research objects, and they have important value in fields such as disease risk stratification, prognosis prediction, and precision medical decision - making. To further standardize this methodology, in 2024, an international multidisciplinary expert group composed of institutions from Switzerland, the Netherlands, the United Kingdom, and others, based on the TRIPOD statement and the PROBAST assessment tool, jointly released the "Step - by - step guide for developing clinical prediction models". This guide systematically constructs 13 steps: defining the objective, creating a team, conducting a literature review, developing a protocol, choosing to develop a new model or update an existing model, defining the outcome measure, identifying candidate predictors, collecting and checking data, determining the sample size, handling missing data, fitting the prediction model, evaluating the performance of the prediction model, determining the final model, performing decision curve analysis, evaluating the predictive ability of individual predictors, writing a report and publishing the results. This paper deeply analyzes the steps of this guide, aiming to provide a reference for clinical researchers.
Objective To construct yeast eukaryotic expression vector carrying human endostatin (ES) cDNA. Methods The functional fragment of endostatin gene in human hepatic tissue was amplified by using RT-PCR technology, and cloned into yeast pPIC9 expression vector. The positive clone was sequenced by using automatized sequencer. Results The endostatin cDNA was successfully cloned. The positive ES clone gene in pPIC9 expression vector was sieved, and its coding sequence was identified to be as same as the previously reported sequence. Conclusion The successful construction of ES gene in pPIC9 expression vector using molecular biological method maybe helpful for the high expression of ES protein, which may lay the foundation for the treatment of malignant tumor through anti-angiogenesis appoach.