The application of virtual simulation technology in the field of teaching has gradually received widespread attention both domestically and internationally. The National Virtual Simulation Experimental Teaching Project Shared Service Platform (iLAB-X) has emerged, providing a good platform and support for the teaching reform of experimental courses in universities. There are many difficulties in traditional experimental teaching of neurobiology. This article combines the teaching content of neurobiology experimental courses, fully explores and utilizes the virtual simulation resources of iLAB-X, and introduces the experimental teaching case design of the virtual real combination mode. It can enable students to have an immersive experience of arcane neurobiological experiments, help to understand and absorb theoretical knowledge, stimulate students’ interest and curiosity, and improve the teaching effectiveness of neurobiology experimental courses.