Effect of MUC1 Over-expression on Chemotherapy of 5-Fluorouracil and Cisplatin for Esophageal Cancer Cells_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

ZHENGMin ^1,2 , YEQing ³ , YANGCheng-guang ² , XUYe ² , GUOMing ² ,  XUZhi-fei ¹

1. Department of Thoracic Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200061, P. R. China;
2. Department of Thoracic Surgery, Shanghai Changning District Central Hospital, Shanghai 200336, P. R. China;
3. Department of Thoracic Surgery, Shanghai Renji Hospital, School of Medicine of Shanghai Jiaotong University, Shanghai 201210, P. R. China;

Corresponding author：

XUZhi-fei, Email: doctorxzf@163.com

Keywords：

Esophageal cancer; MUC1; Cisplatin; 5-Fluorouracil; Nude mice

DOI：

10.7507/1007-4848.20140107

Video：

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Objective To investigate MUC1 over-expression on chemotherapy of 5-fluorouracil and cisplatin for esophageal cancer cells. Methods MUC1 over-expression and stable silencing of MUC1 expression esophageal cancer cell lines were constructed. Xenograft model of esophageal cancer was established in nude mice. Cisplatin (8 mg/kg, day 1 and day 7)and 5-fluorouracil (20 mg/kg, day 1 to 6)were injected intraperitoneally. Tumor volume and body weight of nude mice were measured. Tumor growth curve and body weight curve were drawn, and tumor inhibitory rate was calculated. Results Both cisplatin and 5-fluorouracil suppressed tumor growth of MUC1 over-expression esophageal cancer nude mice. Body weight and tumor volume of nude mice of cisplatin and 5-fluorouracil groups were significantly smaller than those of the control group (P < 0.05), and the inhibitory effects of cisplatin were significantly greater than those of 5-fluorouracil (P < 0.05). There was no significant inhibitory effect in stable silencing of MUC1 expression esophageal cancer nude mice. Conclusion Both cisplatin and paclitaxel can suppress the growth of MUC1 over-expression esophageal cancer, and cisplatin has greater inhibitory effects than 5-fluorouracil in tumor volume and body weight of nude mice.

Citation： ZHENGMin, YEQing, YANGCheng-guang, XUYe, GUOMing, XUZhi-fei. Effect of MUC1 Over-expression on Chemotherapy of 5-Fluorouracil and Cisplatin for Esophageal Cancer Cells. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2014, 21(3): 394-398. doi: 10.7507/1007-4848.20140107 Copy

1.	Creaney J, Segal A, Sterrett G, et al. Overexpression and altered glycosylation of MUCl in malignant mesothelioma. Br J Cancer, 2008, 98 (9):1562-1569.
2.	王立顺, 朱迅. MUC1和MUC2 mRNA在不同肿瘤组织的表达及其意义.中国肿瘤临床, 2000, 27 (4):258-261.
3.	于秀文, 李淑霞, 冯美燕, 等.黏蛋白MUC1和MUC2联合表达与大肠癌的相关性.临床与实验病理学杂志, 2004, 20 (5):611-614.
4.	赵立明, 王永翔, 刘学军, 等.膀胱尿路上皮细胞癌MUC1和MUC2蛋白的表达及临床意义.中国临床医学, 2009, 16 (2):239-241.
5.	Nozoe T, Ezaki T, Kabashima A, et al. Significance of immunohistochemical expression of cyclooxygenase-2 in squamous cell carcinoma of the esophagus. Am J Surg, 2005, 189 (1):110-115.
6.	逄旭光, 范虹, 葛棣. HER4基因抑制对食管癌细胞Eca-109迁移和侵袭能力的影响.复旦学报:医学版, 2008, 35 (4):521-527.
7.	赵璇, 李沛, 马俊芬, 等. TIMP3、E-Cadherin基因在食管癌细胞系EC1和EC9706中的甲基化及蛋白表达.世界华人消化杂志, 2010, 18 (4):379-383.
8.	Feng JG, Liu Q, Qin X, et al. Clinicopathological pattern and Annexin A2 and Cdc42 status in patients presenting with differen tiation and lymphnode metastasis of esophageal squamous cell carcinomas. Mol Biol Rep, 2012, 39 (2):1267-1274.
9.	赵意, 蔡开灿, 刘德纲, 等.恩度联合TP方案对食管癌细胞抑制作用的实验研究.实用医学杂志, 2012, 28 (12):1963-1965.
10.	Guang W, Czinn SJ, Blanchard TG, et al. Genetic regulation of MUC1 expression by Helicobacter pylori in gastric cancer cells. Biochem Biophys Res Commun, 2014, 445 (1)145-150.
11.	Lavrsen K, Madsen CB, Rasch MG, et al. Aberrantly glycosylated MUC1 is expressed on the surface of breast cancer cells and a target for antibody-dependent cell-mediated cytotoxicity. Glycoconj J, 2013, 30 (3):227-236.
12.	Deng JL, Wang L, Chen HM, et al. The role of tumour-asso ciated MUC1 in epithelial ovarian Cancer metastasis and progression. Cancer Metastasis Rev, 2013, 32 (3/4):535-551.
13.	Suzuki Y, Sutoh M, Hatakeyama S, et al. MUC1 carrying core 2 O-glycans functions as a molecular shield against NK cell attack, promoting bladder tumor metastasis. Int J Oncol, 2012, 40 (6):1831-1838.
14.	翁羽蕙, 张铀. MUCl的研究进展.重庆医学, 2010, 39 (10):1288-1290.
15.	Chou Tc. Theoretical basis, experimental design, and compu-terized simulation of synergism and antagonism in drug combination studies. Pharmacol Rev, 2006, 58 (3):621-681.
16.	苏翔宇, 李苏宜, 刘琳.奈达铂联合顺铂对人食管癌细胞株(Eca-109)抑制作用机制的研究.实用肿瘤杂志, 2010, 25 (2):150-155.
17.	Jin Y, Li J, Rong LF, et al. Anti-hepatocarcinoma effects of 5-fluorouracil encapsulated by galactosylceramide liposomes in vivo and in vitro. World J Gastroenterol, 2005, 11 (17):2643-2646.
18.	Abdi K, Khalaj A, Ostad SN, et al. Cytotoxicity and radiosensitising activity of synthesized dinitrophenyl derivatives of 5-fluorouracil. Daru, 2012, 20 (1):3.
19.	Milross CG, Mason KA, Hunter NR, et al. Relationship of mitotic arrest and apoptosis to antitu-mor effete of paelitaxel. Natl Cancer Inst, 1996, 88 (18):1308-1314.
20.	Uguz AC, Cig B, Espino J, et al. Melatonin potentiates chemotherapy-induced cytotoxicity and apoptosis in rat pancreatic tumor cells. J Pineal Res, 2012, 53 (1):91-98.

1. Creaney J, Segal A, Sterrett G, et al. Overexpression and altered glycosylation of MUCl in malignant mesothelioma. Br J Cancer, 2008, 98 (9):1562-1569.
2. 王立顺, 朱迅. MUC1和MUC2 mRNA在不同肿瘤组织的表达及其意义.中国肿瘤临床, 2000, 27 (4):258-261.
3. 于秀文, 李淑霞, 冯美燕, 等.黏蛋白MUC1和MUC2联合表达与大肠癌的相关性.临床与实验病理学杂志, 2004, 20 (5):611-614.
4. 赵立明, 王永翔, 刘学军, 等.膀胱尿路上皮细胞癌MUC1和MUC2蛋白的表达及临床意义.中国临床医学, 2009, 16 (2):239-241.
5. Nozoe T, Ezaki T, Kabashima A, et al. Significance of immunohistochemical expression of cyclooxygenase-2 in squamous cell carcinoma of the esophagus. Am J Surg, 2005, 189 (1):110-115.
6. 逄旭光, 范虹, 葛棣. HER4基因抑制对食管癌细胞Eca-109迁移和侵袭能力的影响.复旦学报:医学版, 2008, 35 (4):521-527.
7. 赵璇, 李沛, 马俊芬, 等. TIMP3、E-Cadherin基因在食管癌细胞系EC1和EC9706中的甲基化及蛋白表达.世界华人消化杂志, 2010, 18 (4):379-383.
8. Feng JG, Liu Q, Qin X, et al. Clinicopathological pattern and Annexin A2 and Cdc42 status in patients presenting with differen tiation and lymphnode metastasis of esophageal squamous cell carcinomas. Mol Biol Rep, 2012, 39 (2):1267-1274.
9. 赵意, 蔡开灿, 刘德纲, 等.恩度联合TP方案对食管癌细胞抑制作用的实验研究.实用医学杂志, 2012, 28 (12):1963-1965.
10. Guang W, Czinn SJ, Blanchard TG, et al. Genetic regulation of MUC1 expression by Helicobacter pylori in gastric cancer cells. Biochem Biophys Res Commun, 2014, 445 (1)145-150.
11. Lavrsen K, Madsen CB, Rasch MG, et al. Aberrantly glycosylated MUC1 is expressed on the surface of breast cancer cells and a target for antibody-dependent cell-mediated cytotoxicity. Glycoconj J, 2013, 30 (3):227-236.
12. Deng JL, Wang L, Chen HM, et al. The role of tumour-asso ciated MUC1 in epithelial ovarian Cancer metastasis and progression. Cancer Metastasis Rev, 2013, 32 (3/4):535-551.
13. Suzuki Y, Sutoh M, Hatakeyama S, et al. MUC1 carrying core 2 O-glycans functions as a molecular shield against NK cell attack, promoting bladder tumor metastasis. Int J Oncol, 2012, 40 (6):1831-1838.
14. 翁羽蕙, 张铀. MUCl的研究进展.重庆医学, 2010, 39 (10):1288-1290.
15. Chou Tc. Theoretical basis, experimental design, and compu-terized simulation of synergism and antagonism in drug combination studies. Pharmacol Rev, 2006, 58 (3):621-681.
16. 苏翔宇, 李苏宜, 刘琳.奈达铂联合顺铂对人食管癌细胞株(Eca-109)抑制作用机制的研究.实用肿瘤杂志, 2010, 25 (2):150-155.
17. Jin Y, Li J, Rong LF, et al. Anti-hepatocarcinoma effects of 5-fluorouracil encapsulated by galactosylceramide liposomes in vivo and in vitro. World J Gastroenterol, 2005, 11 (17):2643-2646.
18. Abdi K, Khalaj A, Ostad SN, et al. Cytotoxicity and radiosensitising activity of synthesized dinitrophenyl derivatives of 5-fluorouracil. Daru, 2012, 20 (1):3.
19. Milross CG, Mason KA, Hunter NR, et al. Relationship of mitotic arrest and apoptosis to antitu-mor effete of paelitaxel. Natl Cancer Inst, 1996, 88 (18):1308-1314.
20. Uguz AC, Cig B, Espino J, et al. Melatonin potentiates chemotherapy-induced cytotoxicity and apoptosis in rat pancreatic tumor cells. J Pineal Res, 2012, 53 (1):91-98.

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