Expressions and Significance on MiR-339-3p and MiR-339-5p of Gastric Carcinoma Cell Lines <i>in Vitro</i>_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HUJie-qiong ,  JINAn-qin , HUANGXiao-jun , YANGLi-hong , JIANGTao

Department of Gastroenterolog, Lanzhou University Sencond Hospital, Lanzhou 730030, Gansu Province, China;

Corresponding author：

JINAn-qin, Email: jinanqin@163.com

Keywords：

MiR-339-3p; MiR-339-5p; Gastric cancer cell; Proliferation; Apoptosis

DOI：

10.7507/1007-9424.20140292

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To study the expression levels of miR-339-3p and miR-339-5p in the gastric carcinoma cell lines(SGC-7901, BGC-823, and MKN-45) and gastric surface epithelium(GES-1);detect the relationship between miR-339-3p and miR-339-5p and the gastric carcinoma cell lines in vetrio experiment through the gain of function, and further significance is suggested. Methods SYBR greenⅠreal time PCR was performed to access the expression of miR-339-3p and miR-339-5p in different cell lines(SGC-7901, BGC-823, MKN-45, and GES-1). The expression levels of miR-339-3p and miR-339-5p were verified by real time PCR experiment again after transfecting miR-339-3p mimics and miR-339-5p mimics. After that, the changes of MKN-45 cells apoptosis and proliferation at 72 h after transfection were detected by flow cytometry and CCK-8 method. Results The expression levels of miR-339-3p and miR-339-5p in gastric carcinoma cell lines(SGC-7901, BGC-823, and MKN-45) were down regulated. Compared with the control group, the apoptosis of MKN-45 cell line was significantly higher(P < 0.05), the ability of proliferation of MKN-45 cell line decreased after transfecting miR-339-3p mimics and miR-339-5p mimics within 72 hours(P < 0.01). Conclusion The expression levels of miR-339-3p and miR-339-5p significantly decreased in the gastric carcinoma cell lines(SGC-7901, BGC-823, and MKN-45) in contrast with gastric surface epithelium. MiR-339-3p and miR-339-5p may be involved in the apoptosis and proferation of the gastric carcinoma.

Citation： HUJie-qiong, JINAn-qin, HUANGXiao-jun, YANGLi-hong, JIANGTao. Expressions and Significance on MiR-339-3p and MiR-339-5p of Gastric Carcinoma Cell Lines in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(10): 1216-1221. doi: 10.7507/1007-9424.20140292 Copy

1.	彭伟, 杨韵, 刘韵霄, 等. microRNA在结直肠癌中的研究及应用进展[J].中国普外基础与临床杂志, 2013, 20(6):691-696.
2.	Gessert S, Bugner V, Tecza A, et al. FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development[J]. Dev Biol, 2010, 341(1):222-235.
3.	Ting Y, Medina DJ, Strair RK, et al. Differentiation-associated miR-22 represses Max expression and inhibits cell cycle progression[J]. Biochem Biophys Res Commun, 2010, 394(3):606-611.
4.	Tsukamoto Y, Nakada C, Noguchi T, et al. MicroRNA-375 is downregulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3zeta[J]. Cancer Res, 2010, 70(6):2339-2349.
5.	Dugas JC, Cuellar TL, Scholze A, et al. Dicer1 and miR-219 are required for normal oligodendrocyte differentiation and myelination[J]. Neuron, 2010, 65(5):597-611.
6.	陈桦, 王小琦, 梁琼.微小RNA与癌症的诊断、治疗及转移[J].兰州大学学报:医学版, 2009, 35(4):82-86.
7.	Callin GA, Seviqnani C, Dumitru CD, et al. Human micorRNA genes are frequently located at fragile sites and genomic regions involved in cancers[J]. Proc Natl Acad Sci USA, 2004, 101(9):2999-3004.
8.	Murakami Y, Yasuda T, Saigo K, et al. Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues[J]. Oncogene, 2006, 25(17):2537-2545.
9.	Iorio MV, Ferracin M, Liu CG, et al. MicroRNA gene expression deregulation in human breast cancer[J]. Cancer Res, 2005, 65(16):7065-7070.
10.	Cummins JM, He Y, Leary RJ, et al. The colorectal micro-RNAome[J]. Proc Natl Acad Sci U S A, 2006, 103(10):3687-3692.
11.	Calin GA, Dumitru CD, Shimizu M, et al. Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia[J]. Proe Natl Acad Sci USA, 2002, 99(24):15524-15529.
12.	Prueitt RL, Yi M, Hudson RS, et al. Expression of microRNAs and protein-coding genes associated with perineural invasion in prostate cancer[J]. Prostate, 2008, 68(11):1152-1164.
13.	Li T, Li D, Sha J, et al. MicroRNA-21 directly targets MARCKS and promotes apoptosis resistance and invasion in prostate cancer cells[J]. Biochem Biophys Res Commun, 2009, 383(3):280-285.
14.	Hayashita Y, Osada H, Tatematsu Y, et al. A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation[J]. Cancer Res, 2005, 65(21):9628-9632.
15.	He H, Jazdewski K, Li W, et al. The role of microRNA genes in papillary thyroid carcinoma[J]. Proc Natl Acad Sci USA, 2005, 102(52):19075-19080.
16.	Guo J, Miao Y, Xiao BX, et al. Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues[J]. J Gastroenterol Hepat, 2009, 24(4):652-657.
17.	Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J]. Cell, 1993, 75(5):843-854.
18.	Li H, Kloosterman W, Fekete DM. MicroRNA-183 family members regulate sensorineural fates in the inner ear[J]. J Neurosci, 2010, 30(9):3254-3263.
19.	Gessert S, Bugner V, Tecza A, et al. FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development[J]. Dev Biol, 2010, 341(1):222-235.
20.	Lin SC, Liu CJ, Lin JA, et al. miR-24 up-regulation in oral carcinoma:positive association from clinical and in vitro analysis[J]. Oral Oncol, 2010, 46(3):204-208.
21.	Asangani IA, Rasheed SA, Nikolova DA, et al. MicroRNA-1(miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer[J]. Oncogene, 2008, 27(15):2128-2136.
22.	Tie J, Pan YL, Zhao LN, et al. MiR-218 inhibits invasion and metastasis of gastric cancer by targeting the robol receptor[J]. PLoS Genet, 2010, 6(3):e1000879.
23.	Blenkiron C, Miska EA. miRNAs in cancer:approaches, aetioloy, diagnostics and therapy[J]. Hum Mol Genet, 2007, 16(l):R106-R113.
24.	蒋振, 郭俊明, 肖丙秀.微小RNA与胃癌关系的研究进展[J].中华病理学杂志, 2008, 37(8):558-560.
25.	Zhang ZY, Li ZJ, Gao CP, et al. MiR-21 plays a pivotal role in gastric cancer pathogenesis and progression[J]. Lab Invest, 2008, 88(12):1358-1366.
26.	Wu ZS, Wu Q, Wang CQ, et al. MiR-339-5p inhibits breast cancer cell migration and invasion in vitro and may be a potential biomarker for breast cancer prognosis[J]. BMC Cancer, 2010, 10:542.

1. 彭伟, 杨韵, 刘韵霄, 等. microRNA在结直肠癌中的研究及应用进展[J].中国普外基础与临床杂志, 2013, 20(6):691-696.
2. Gessert S, Bugner V, Tecza A, et al. FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development[J]. Dev Biol, 2010, 341(1):222-235.
3. Ting Y, Medina DJ, Strair RK, et al. Differentiation-associated miR-22 represses Max expression and inhibits cell cycle progression[J]. Biochem Biophys Res Commun, 2010, 394(3):606-611.
4. Tsukamoto Y, Nakada C, Noguchi T, et al. MicroRNA-375 is downregulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3zeta[J]. Cancer Res, 2010, 70(6):2339-2349.
5. Dugas JC, Cuellar TL, Scholze A, et al. Dicer1 and miR-219 are required for normal oligodendrocyte differentiation and myelination[J]. Neuron, 2010, 65(5):597-611.
6. 陈桦, 王小琦, 梁琼.微小RNA与癌症的诊断、治疗及转移[J].兰州大学学报:医学版, 2009, 35(4):82-86.
7. Callin GA, Seviqnani C, Dumitru CD, et al. Human micorRNA genes are frequently located at fragile sites and genomic regions involved in cancers[J]. Proc Natl Acad Sci USA, 2004, 101(9):2999-3004.
8. Murakami Y, Yasuda T, Saigo K, et al. Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues[J]. Oncogene, 2006, 25(17):2537-2545.
9. Iorio MV, Ferracin M, Liu CG, et al. MicroRNA gene expression deregulation in human breast cancer[J]. Cancer Res, 2005, 65(16):7065-7070.
10. Cummins JM, He Y, Leary RJ, et al. The colorectal micro-RNAome[J]. Proc Natl Acad Sci U S A, 2006, 103(10):3687-3692.
11. Calin GA, Dumitru CD, Shimizu M, et al. Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia[J]. Proe Natl Acad Sci USA, 2002, 99(24):15524-15529.
12. Prueitt RL, Yi M, Hudson RS, et al. Expression of microRNAs and protein-coding genes associated with perineural invasion in prostate cancer[J]. Prostate, 2008, 68(11):1152-1164.
13. Li T, Li D, Sha J, et al. MicroRNA-21 directly targets MARCKS and promotes apoptosis resistance and invasion in prostate cancer cells[J]. Biochem Biophys Res Commun, 2009, 383(3):280-285.
14. Hayashita Y, Osada H, Tatematsu Y, et al. A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation[J]. Cancer Res, 2005, 65(21):9628-9632.
15. He H, Jazdewski K, Li W, et al. The role of microRNA genes in papillary thyroid carcinoma[J]. Proc Natl Acad Sci USA, 2005, 102(52):19075-19080.
16. Guo J, Miao Y, Xiao BX, et al. Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues[J]. J Gastroenterol Hepat, 2009, 24(4):652-657.
17. Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J]. Cell, 1993, 75(5):843-854.
18. Li H, Kloosterman W, Fekete DM. MicroRNA-183 family members regulate sensorineural fates in the inner ear[J]. J Neurosci, 2010, 30(9):3254-3263.
19. Gessert S, Bugner V, Tecza A, et al. FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development[J]. Dev Biol, 2010, 341(1):222-235.
20. Lin SC, Liu CJ, Lin JA, et al. miR-24 up-regulation in oral carcinoma:positive association from clinical and in vitro analysis[J]. Oral Oncol, 2010, 46(3):204-208.
21. Asangani IA, Rasheed SA, Nikolova DA, et al. MicroRNA-1(miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer[J]. Oncogene, 2008, 27(15):2128-2136.
22. Tie J, Pan YL, Zhao LN, et al. MiR-218 inhibits invasion and metastasis of gastric cancer by targeting the robol receptor[J]. PLoS Genet, 2010, 6(3):e1000879.
23. Blenkiron C, Miska EA. miRNAs in cancer:approaches, aetioloy, diagnostics and therapy[J]. Hum Mol Genet, 2007, 16(l):R106-R113.
24. 蒋振, 郭俊明, 肖丙秀.微小RNA与胃癌关系的研究进展[J].中华病理学杂志, 2008, 37(8):558-560.
25. Zhang ZY, Li ZJ, Gao CP, et al. MiR-21 plays a pivotal role in gastric cancer pathogenesis and progression[J]. Lab Invest, 2008, 88(12):1358-1366.
26. Wu ZS, Wu Q, Wang CQ, et al. MiR-339-5p inhibits breast cancer cell migration and invasion in vitro and may be a potential biomarker for breast cancer prognosis[J]. BMC Cancer, 2010, 10:542.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions and Significance on MiR-339-3p and MiR-339-5p of Gastric Carcinoma Cell Lines in Vitro

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content