Improve The Survival and Function of Islets by Combined Transplantation of Human Endothelial Cells in Peripheral Blood and Islet of Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LUOWei ¹ , LIWei-ye ² , QINHong-jun ¹ , HURen-jian ¹ , HUHong-qiang ¹ ,  LIAng ²

1. Department of The Military Ward, Sichuan People's Armed Police Corps Hospital, Leshan 614000, Sichuan Province, China;
2. Department of Hepatobiliary and Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China;

Corresponding author：

LIAng, Email: angli1979@hotmail.com

Keywords：

Endothelial cells in peripheral blood; Islet transplantation; Survival; Nude mice

DOI：

10.7507/1007-9424.20140293

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore the endothelial cells from human peripheral blood and islet of rat co-transplantation under the renal capsule of diabetic nude mice to improve the survival and function of transplanted islet. Methods The endothelial cells from human peripheral blood(5×10⁵)and freshly isolated rat islet cells were co-transplanted under the renal capsule of diabetic nude mice model, then the fasting blood glucose, body weight, peripheral blood C-peptide level, and intraperitoneal glucose tolerance test(IPGTT) were measured to evaluated the islet graft survival and function. Results Compared with the control group, the fasting blood glucose level significantly decreased(P < 0.01), peripheral blood C-peptide level rised(P < 0.01), and body weight increased(P < 0.01) of receptor nude mice in experience group, the IPGTT also improved. Conclusion The endothelial cells from human peripheral blood and islet of rat co-transplan-tation can obviously improve the survival and function of transplanted islet of nude mice.

Citation： LUOWei, LIWei-ye, QINHong-jun, HURen-jian, HUHong-qiang, LIAng. Improve The Survival and Function of Islets by Combined Transplantation of Human Endothelial Cells in Peripheral Blood and Islet of Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(10): 1222-1225. doi: 10.7507/1007-9424.20140293 Copy

1.	Shapiro AM, Lakey JR, Ryan EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen[J]. N Engl J Med, 2000, 343(4):230-238.
2.	Barton FB, Rickels MR, Alejandro R, et al. Improvement in outcomes of clinical islet transplantation:1999-2010[J]. Diabetes Care, 2012, 35(7):1436-1445.
3.	Shapiro AM. State of the art of clinical islet transplantation and novel protocols of immunosuppression[J]. Curr Diab Rep, 2011, 11(5):345-354.
4.	Cantarelli E, Melzi R, Mercalli A, et al. Bone marrow as an alternative site for islet transplantation[J]. Blood, 2009, 114(20):4566-4574.
5.	Angaswamy N, Tiriveedhi V, Banan B, et al. Synergism of a natural plant product, oleanolic acid with calcineurin inhibitor in prolonging islet allograft survival[J]. Transpl Immunol, 2013, 29(1-4):64-70.
6.	Biarnés M, Montolio M, Nacher V, et al.β-cell death and mass in syngeneically transplanted islets exposed to short-and long-term hyperglycemia[J]. Diabetes, 2002, 51(1):66-72.
7.	Keymeulen B, Gillard P, Mathieu C, et al. Correlation betweenβcell mass and glycemic control in type 1 diabetic recipients of islet cell graft[J]. Proc Natl Acad Sci U S A, 2006, 103(46):17444-17449.
8.	Song HJ, Xue WJ, Li Y, et al. Prolongation of islet graft survival using concomitant transplantation of islets and vascular endothelial cells in diabetic rats[J]. Transplant Proc, 2010, 42(7):2662-2665.
9.	Kang S, Park HS, Jo A, et al. Endothelial progenitor cell cotransplantation enhances islet engraftment by rapid revascularization[J]. Diabetes, 2012, 61(4):866-876.
10.	Ward MR, Stewart DJ, Kutryk MJ. Endothelial progenitor cell therapy for the treatment of coronary disease, acute MI, and pulmonary arterial hypertension:current perspectives[J]. Catheter Cardiovasc Interv, 2007, 70(7):983-998.
11.	Kawamoto A, Losordo DW. Endothelial progenitor cells for cardiovascular regeneration[J]. Trends Cardiovasc Med, 2008, 18(1):33-37.
12.	Xu QB. Endothelial progenitor cells in angiogenesis[J].生理学报, 2005, 57(1):1-6.
13.	Werner N, Kosiol S, Schiegl T, et al. Circulating endothelial progenitor cells and cardiovascular outcomes[J]. N Engl J Med, 2005, 353(10):999-1007.
14.	Yoder MC, Mead LE, Prater D, et al. Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals[J]. Blood, 2007, 109(5):1801-1809.
15.	Moubarik C, Guillet B, Youssef B, et al. Transplanted late outgrowth endothelial progenitor cells as cell therapy product for stroke[J]. Stem Cell Rev, 2011, 7(1):208-220.
16.	Hendrickx B, Verdonck K, Van den Berge S, et al. Integration of blood outgrowth endothelial cells in dermal fibroblast sheets promotes full thickness wound healing[J]. Stem Cells, 2010, 28(7):1165-1177.
17.	侯凌, 尹注增, 向莹, 等.大鼠胰岛的分离和纯化[J].华中科技大学学报, 2009, 38(3):413-416.
18.	郭新宾, 崔维韵, 刘庆国, 等.人外周血内皮祖细胞分离培养与鉴定[J].中国现代神经疾病杂志, 2009, 9(2):159-163.
19.	Pan X, Xue W, Li Y, et al. Islet graft survival and function:concomitant culture and transplantation with vascular endothelial cells in diabetic rats[J]. Transplantation, 2011, 92(11):1208-1214.
20.	Limmer A, Ohl J, Kurts C, et al. Efficient presentation of exog-enous antigen by liver endothelial cells to CD8⁺ T cells results in antigen-specific T-cell tolerance[J]. Nat Med, 2000, 6(12):1348-1354.
21.	Sotiropoulou PA, Papamichail M. Immune properties of mesenc-hymal stem cells[J]. Methods Mol Biol, 2007, 407:225-243.

1. Shapiro AM, Lakey JR, Ryan EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen[J]. N Engl J Med, 2000, 343(4):230-238.
2. Barton FB, Rickels MR, Alejandro R, et al. Improvement in outcomes of clinical islet transplantation:1999-2010[J]. Diabetes Care, 2012, 35(7):1436-1445.
3. Shapiro AM. State of the art of clinical islet transplantation and novel protocols of immunosuppression[J]. Curr Diab Rep, 2011, 11(5):345-354.
4. Cantarelli E, Melzi R, Mercalli A, et al. Bone marrow as an alternative site for islet transplantation[J]. Blood, 2009, 114(20):4566-4574.
5. Angaswamy N, Tiriveedhi V, Banan B, et al. Synergism of a natural plant product, oleanolic acid with calcineurin inhibitor in prolonging islet allograft survival[J]. Transpl Immunol, 2013, 29(1-4):64-70.
6. Biarnés M, Montolio M, Nacher V, et al.β-cell death and mass in syngeneically transplanted islets exposed to short-and long-term hyperglycemia[J]. Diabetes, 2002, 51(1):66-72.
7. Keymeulen B, Gillard P, Mathieu C, et al. Correlation betweenβcell mass and glycemic control in type 1 diabetic recipients of islet cell graft[J]. Proc Natl Acad Sci U S A, 2006, 103(46):17444-17449.
8. Song HJ, Xue WJ, Li Y, et al. Prolongation of islet graft survival using concomitant transplantation of islets and vascular endothelial cells in diabetic rats[J]. Transplant Proc, 2010, 42(7):2662-2665.
9. Kang S, Park HS, Jo A, et al. Endothelial progenitor cell cotransplantation enhances islet engraftment by rapid revascularization[J]. Diabetes, 2012, 61(4):866-876.
10. Ward MR, Stewart DJ, Kutryk MJ. Endothelial progenitor cell therapy for the treatment of coronary disease, acute MI, and pulmonary arterial hypertension:current perspectives[J]. Catheter Cardiovasc Interv, 2007, 70(7):983-998.
11. Kawamoto A, Losordo DW. Endothelial progenitor cells for cardiovascular regeneration[J]. Trends Cardiovasc Med, 2008, 18(1):33-37.
12. Xu QB. Endothelial progenitor cells in angiogenesis[J].生理学报, 2005, 57(1):1-6.
13. Werner N, Kosiol S, Schiegl T, et al. Circulating endothelial progenitor cells and cardiovascular outcomes[J]. N Engl J Med, 2005, 353(10):999-1007.
14. Yoder MC, Mead LE, Prater D, et al. Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals[J]. Blood, 2007, 109(5):1801-1809.
15. Moubarik C, Guillet B, Youssef B, et al. Transplanted late outgrowth endothelial progenitor cells as cell therapy product for stroke[J]. Stem Cell Rev, 2011, 7(1):208-220.
16. Hendrickx B, Verdonck K, Van den Berge S, et al. Integration of blood outgrowth endothelial cells in dermal fibroblast sheets promotes full thickness wound healing[J]. Stem Cells, 2010, 28(7):1165-1177.
17. 侯凌, 尹注增, 向莹, 等.大鼠胰岛的分离和纯化[J].华中科技大学学报, 2009, 38(3):413-416.
18. 郭新宾, 崔维韵, 刘庆国, 等.人外周血内皮祖细胞分离培养与鉴定[J].中国现代神经疾病杂志, 2009, 9(2):159-163.
19. Pan X, Xue W, Li Y, et al. Islet graft survival and function:concomitant culture and transplantation with vascular endothelial cells in diabetic rats[J]. Transplantation, 2011, 92(11):1208-1214.
20. Limmer A, Ohl J, Kurts C, et al. Efficient presentation of exog-enous antigen by liver endothelial cells to CD8⁺ T cells results in antigen-specific T-cell tolerance[J]. Nat Med, 2000, 6(12):1348-1354.
21. Sotiropoulou PA, Papamichail M. Immune properties of mesenc-hymal stem cells[J]. Methods Mol Biol, 2007, 407:225-243.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Improve The Survival and Function of Islets by Combined Transplantation of Human Endothelial Cells in Peripheral Blood and Islet of Rats

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content