目的 研究结直肠癌患者血清中肝细胞生长因子(hepatocyte growth factor,HGF)的表达水平及其与结直肠癌肝转移的关系。 方法 收集我院2006年9月至2009年1月期间手术治疗并经病理证实为结直肠癌患者35例，应用夹心ELISA法检测血清中HGF表达水平。 结果 血清中HGF表达在结直肠癌有远处转移者中为(0.37±0.11) ng/ml，明显高于无转移者的(0.20±0.06) ng/ml (Plt;0.05)； T3、T4期者为(0.32±0.29) ng/ml，亦明显高于T1、T2期者的(0.20±0.14) ng/ml (Plt;0.05)； 肝转移者为(0.43±0.10) ng/ml，明显高于其他部位转移者的(0.31±0.09) ng/ml (Plt;0.05)。 结论 血清HGF水平与结直肠癌发展及肝转移发生有密切关系,血清HGF水平有望作为监测结直肠癌转移的临床指标。
Cytogenetic study of 18 colorectal carcinomas confirmed the extensive heterogeneity and the complexity of the karyotypic picture in this tumor.Karyotypic analysis showed that chromosomes 7 and 3 were of the highest chromosomal gaining frequencies(72%,66%) and chromosomal losses were shown in chromosome 17(50%),chromosome5(44%) and chromosome 18(33%).The structual rearrangements frequently involved were 17p(78%),5q(61%),6q,7q,8p,12q,2p,etc.A great number of marker chromosomes and polyploid chromosomes had bad prognosis relatively.According to these results,we conclude that chromosomes 17,5,and 18 may play an important role in the evolution of colorectal cancer.
Objective To discuss the safety and clinical differences among elderly people being treated with neo-adjuvant chemotherapy combined with operation. Methods To analyze retrospectively the data of patients diagnosed definitely as colorectal cancer in West China Hospital of Sichuan University from June 2007 to December 2007, and to compare the clinical effect differences between elderly group (≥60 years) and non-elderly group (lt;60 years) with combined therapy. Results Among preoperative indexes, the constituent ratios of elderly group combined with cardiac vessels system diseases, endocrine system diseases or other system diseases were all obviously higher than those of non-elderly group, the differences showed statistical significance (Plt;0.05); Constituent ratio of elderly patients with hypertension or diabetes mellitus was also higher than that of non-elderly patients, there were still statistical significance (Plt;0.05), but no statistical significances among other preoperative indexes (Pgt;0.05); Differences of indexes during operation between two groups showed no statistical significance (Pgt;0.05). But during postoperative indexes, complications in elderly group were higher than those of non-elderly group, and the differences had statistical significance (Plt;0.05), and the time of removing gastric canal, urinary catheter and drainage tube between two groups showed no statistical significance (Pgt;0.05); Meanwhile, the intake time, venting time and defecation time after operation and out-of-bed activity time after operation also had no statistical significance (Pgt;0.05). As to the adverse effect relate to chemotherapy, there were no statistical significances (Pgt;0.05). Conclusion Elderly patients may have more poor physical condition and it maybe more difficult to treat them. In spite of this, the comprehensive treatment of neo-adjuvant chemotherapy combined with surgical operation will not lead to the delay of surgical therapy, in addition, operating modus during surgical intervention will not be effected and its therapeutic effect in the near future was optimistic. So the application of this combined therapy has some clinical effects and would be safety.
ObjectiveTo summarize the current advancement of the relationship between dietary factors and colorectal cancer in recent years, looking for more reasonable prevention measures. MethodsRelated literatures about the relationship between diet composition and the occurrence of colorectal cancer at home and abroad were collected to make a review. ResultsAmong the environmental factors, dietary factors had a close relationship with colorectal cancer, and was easy to control. Nowadays, it was believed that high animal fat, excessive alcohol consumption, consumption of fried, barbecue, and pickles may be positively associated with the occurrence of colorectal cancer. However, high fiber, high vitamin, high folic acid, the amount of minerals and trace elements in diet were negatively correlated with the occurrence of colorectal cancer. ConclusionsColorectal cancer occurs by environmental factors, genetic factors, and other factors. Diet model of people in different areas is not the same, the occurrence rate of colorectal cancer is also different, so it needs further study to explore its mechanism.
Objective To explore the factors associated with clinic follow-up of old patients with colorectal cancer and provide more evidence to improve the efficiency and quality of clinic follow-up after sugery. Methods The data of 253 patients who were underwent sugery because of old colorectal cancer in our hospital from January 2009 to May 2010 were reviewed. Data about the rate and times of clinic follow-up within 6 months after operation were collected via Hospital Information Systerm, then the follow-up rate was calculated, and to analyse the possible factors associated with follow-up times and rate. Results The total follow-up rate was 84.2%（213/253）, and the total times of follow-up was between 0 to 24 times per one, （4.08±0.03）times on average. On the times of follow-up, patients inside the city was higher than that outside, patients with medical or postoperative complications were higher than those without, and patients with a stoma was lower than that without, and the differences were statistically significant （P＜0.05）.While on the follow-up rate, patients underwent a radical sugery or with a stoma were lower than those not or without, and the differences were statistically significant （P＜0.05）. Conclusions The clinic follow-up of old colorectal cancer patients is not satisfactory, and the possible factors associated with follow-up times or rate are the distance between residence and hospital, have medical complications or not, have postoperative complications or not, radical sugery or not, and with a stoma or without.
【Abstract】ObjectiveTo detect the expression of human papilloma virus(HPV) 16 E7 was detected in colorectal adenocarcinoma tissue and normal mucosa. MethodsEighty-two patients with primary colorectal adenocarcinoma were selected in this study. The samples were taken from the tumor and the adjacent normal mucosa (10 cm away from the tumor) in each patient. Polymerase chain reaction (PCR) and immunohistochemistry were used to detect HPV16 E7 DNA and protein respectively. ResultsHPV16 E7 DNA expression was significantly higher in colorectal carcinoma (51.22%,42/82) than that in adjacent normal mucosa (4.88%,4/82), P＜0.01. A correlation was found between HPV16 E7 DNA expression and tumor location (P＜0.05)，18.18% in the ascending colon carcinoma and 64.10% in the rectal carcinoma. HPV16 E7 DNA expression was also associated with Dukes stage（P＜0.01）, but was not correlated with cancer differentiation. HPV16 E7 protein expression was mainly dectected in the nuclei of tumor cells with immunohistochemistry. There was a correlation between the expression of HPV16 E7 protein and HPV16 E7 gene. PCR had a higher sensitivity than immunohistochemistry. ConclusionHPV16 infection rate is much higher in the colorectal carcinoma than that in the adjacent normal mucosa, which indicates that HPV16 infection exists in some colorectal carcinomas. The high infection rate of HPV16 E7 is associated with advanced Dukes stage and proximity to anus.