The DNA content, cellular ultrastructure and the expression of blood group Y antigen and immunosuppressive acidic protein-2(IAP-2) were observed in normal breast, cystic hyperplasia of breast and breast cancer. The results showed: the results observed in the cells of cystic hyperplasia with epithelial proliferation grade Ⅰ were similar to those in normal breast cells. The DNA content increased, the hypoplasia and dedifferentiation features in some structures of cellular membrane and nucleus were observed, and the abnormal antigens expressed in part of the atypical hyperplasic cells. The DNA content and ultrastructure in a part of cells with aypical hyperplasia grade Ⅲ were similar to those in the cells of breast cancer grade Ⅰ. The results indicated that in the couse of atypical hyperplasia, the biological abnormalities and its extent of those cells were closely related to the differentiation extent, the developing tendency and the risk of canceration of the cystic hyperplasia of breast.
The aim of the this study was to search for bacterial DNA sequences in cholesterol gallstones with negative bacterial culture by NP-PCR technique. Bacterial gene fragments were amplified in vitro from DNA which were extracted from cholesterol gallstones in gallbladder for identifying the existence of bacteria. The gallbladder gallstones of 30 patients were analysed. Bacterial DNA was found in the stones of 26 patients, indicating that most cholesterol gallstones harbor bacterial DNA.
ObjectiveTo explore the influence of paternal serum HBV-DNA load levels and pregnant women's HBsAb on vertical transmission of hepatitis B virus (HBV) from HBsAg positive fathers to infants in order to provide effective methods for paternal-fetal ventrical transmission of HBV prevention. MethodsUsing HBsAg and HBV-DNA as indicators to screen pregnant women and their husbands after gained consent, 121 families with HBVM negative or only HBsAb positive and HBV-DNA negative pregnant women, HBsAg positive husbands and their newborns were selected. In this case-control study, according to neonatal cord blood HBV-DNA detection, 23 newborns with cord blood HBV-DNA positive were selected as cases, 98 newborns as controls. ResultsThe positive rate of neonatal cord blood HBV-DNA was 19.0% (23/121); and there was dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive (trend χ2=60.108, P=0.000). The analysis of ROC curve showed that paternal serum HBV-DNA load level (106 copies/mL) is a better demarcation point to forecast the occurrence of vertical transmission of HBV from HBsAg positive fathers to infants, because there was a better sensitivity and specificity during forecast; and HBsAb negative pregnant women's were statistically significant (χ2=12.399, P=0.000). There was no significant difference at the positive rate of neonatal cord blood HBV-DNA between the case group and control group when paternal serum HBV-DNA load levels exceed 107 copies/mL (P > 0.05). ConclusionPaternal serum HBV-DNA load levels and HBsAb negative pregnant women are the risk factors of vertical transmission of HBV from HBsAg positive father to infants. Paternal serum HBV-DNA load level (106 copies/mL) is an appropriate index of the occurrence of vertical transmission.
To find the relation between the damage of gastric remnant mucosal barrier and the precancerous lesion of gastric remnant mucosa, in the process of the canine gastric remnant precarcinogenesis induced by N-methyN’-nitro-N-nitrosoguanidine (MNNG), we performed regularly the esophagogastroscopy and the mucosal biopsy.At the same time, we also measured gastric transmucosal potential difference and intracellular DNA content of remnant mucosa.We found that the more severe the damage of gastric remnant mucosal barrier was , the greater the malignant capacity of gastric remnant mucosal was.Our study suggests that the damage of gastric remnant mucosal barrier plays an important role in the gastric remnant mucosal precarcinogenesis.
The oncogene ras p21 expression and DNA content in 46 cases of colorectal tumor were analysed quantitatively with flow cytometry and cyto-immunofluorescence staining technique. The results showed that the positive rate of ras p21 expression was 65.7% and the rate of DNA aneuploid was 74.3% in colorectal carcinomas. Ras p21 expression was higher in colorectal adenocarcinomas than that of the adenomas and normal mucosa. DNA ploid and proliferative index had some association with ras p21 expresssion. Detection of ras p21 expression and DNA content in tumors may be helpful in predicting the outcome of colorectal cancer patients.
Porpose To investigate the optimal concentration of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on DNA synthesis and their synergism indensity arrested human retinal pigment epithelial (RPE) cells. Methods Growth factor effects in cultured human RPE of the 6th generation were assessed by [3 H]-thymidine incorporation and radioautography. Results EGF and bFGF were potent stimulators when used alone,and their optimal concentrations were 10ng/ml in DMEM and 1ng/ml in 2% serum DMEM.When used in combination (10ng/ml EGF and 10ng/ml bFGF),they caused a significant enhancement of [3 H]-thymidine incorporation about 2.96 times. Conclusion EGF and bFGF were potent stimulators in RPE cells,and demonstrated synergism in their action. (Chin J Ocul Fundus Dis,1998,14:98-100)
Dysplasia of gastric stump mucosa in 47 cases was studies.Nuclear DNA contents were measured with an automatic imagie analysis system.The results showed that the mean values of the nuclear DNA contents,area,perimeter,maximum diameter,minimum diameter increased with the increase of severity of dysplasia in gastric stump mucosa(Plt;0.01);where as nuclear form factor decreased with the increase of severity of dysplasia in gastric stump mucosa(Plt;0.05).Severe dysplasia is similar to that of gastric stump cancer in the DNA ploidy histogram.Our results indicate biological behaviour of gastric stump mucosa dysplasia.This study suggests that DNA contents analysis may be used as an important reference for grading,screening,and treating dysplasia of gastric stump mucosa.
Ｂｙ ｕｓｉｎｇ ｍｅｄｉｃａｌ ｉｍａｇｅ ｐｒｏｃｅｓｓ ｓｙｓｔｅｍ ｆｏｒ ＤＮＡ ｃｏｎｔｅｎｔｓ，２６ ｃａｓｅｓ ｏｆ ｔｈｙｒｏｉｄ ｔｕｍｏｒｓ ｔｈａｔ ｃｏｍｐｒｉｓｅｄ ａｄｅｎｏｍａ ５，ｐａｎｉｌｌａｒｙ ａｄｅｎｏｃａｒｃｉｎｏｍａ １４，ｆｏｌｌｉｃｕｌａｒ ｃａｒｃｉｎｏｍａ ５，ｕｎｄｉｆｆｅｒｅｎｔｉａｔｅｄ ｃａｒｃｉｎｏｍａ ２ ａｎｄ ｎｏｒｍａｌ ｔｈｙｒｏｉｄ ｔｉｓｓｕｅ ３ ｗｅｒｅ ｄｅｔｅｃｔｅｄ．Ｔｈｅ ＤＮＡ ｃｏｎｔｅｎｔｓ ｉｎ ｔｈｅ ｎｕｍｂｅｒ ｏｆ ｐｏｌｙｐｌｏｉｄｙ ｗｅｒｅ：ｃａｒｃｉｎｏｍａ ｔｈｅ ｈｉｇｈｅｓｔ ｉｎ ａｍｏｕｎｔ，ａｄｅｎｏｍａ ｔｈｅ ｍｅｄｉｕｍ ａｎｄ ｎｏｒｍａｌ ｔｈｙｒｏｉｄ ｔｉｓｓｕｅ ｔｈｅ ｌｅａｓｔ，ｈｅｎｃｅ ｗｅ ｐｒｏｐｏｓｅ ｔｈａｔ ｔｈｅ ｄｅｔｅｒｍｉｎａｔｉｏｎ ｏｆ ＤＮＡ ｐｌｏｉｄｙ ｉｎ ｔｈｙｒｏｉｄ ｔｕｍｏｒｓ ｍａｙ ｂｅ ｕｓｅｄ ａｓ ａｎ ａｄｊｕｖａｎｔ ｔｏ ｅｖａｌｕａｔｅ ｔｈｅ ｐｒｏｌｉｆｅｒａｔｉｖｅ ａｃｔｉｖｉｔｙ ｏｆ ｔｈｙｒｏｉｄ ｔｕｍｏｒ．