Objective To explore the effect of different nutrition on the immune function of patients with colorectal cancer. Methods A total of 640 colorectal cancer patients were randomized into the enteral nutrition and peripheral nutrition groups. The peripheral venous blood was sampled before operation and on the postoperative days 1 and 7 to determine the serum levels of IgG, IgA, IgM, the percentage of T lymph cell subgroups CD3, CD4, CD4/CD8 and CRP, PGE, α- antitrypsin etc. The patient’s general condition, side-effects, and complications were observed intimately after surgery. Results Compared with the control group, the IgG, IgA, IgM, CD3, CD4,CD4/CD8 levels of the trial group on postoperative day 7 were higher. But the levels of CRP, PGE, α- antitrypsin were decreased. Hospitalization time and anal exsufflation time were shorter as well. There was no significant difference in either the general conditions or complications between the two groups. Conclusion Application of enteral nutrition after colorectal cancer surgery is safe, ,effective, and can improve the patient’s immune function and prognosis.
To study the effects of arginine supplementation in total parenteral nutrition (TPN) on lymphocytic immune function in postoperative patients with gastric cancer. Thirty six patients with gastric cancer receiving TPN were eligible for entry into randomized and prospective clinical trial of the study. T cell subsets, NK cell activity, plasma IL-2 content and peripheral blood CD25 were measured in before and after parenteral nutrition of the patients. Results: usual TPN could not improve lymphocytic immunosuppression of postoperative patients with gastric cancer. The patients receiving arginine supplementation in TPN might enhance lymphocytic immune function by increasing CD4 level, IL-2 production and NK cell activity, but there was no significant effect of arginine on CD25 expression. Conclusion: there are some effects of supplement with arginine on releasing of the cellular immunosuppression and restoring of lymphocytic immune function.
Objective To detect the expression of cytokeratin 20 (CK20) mRNA (micrometastasis) in regional lymph nodes and the serum activities of CD4+ cells, CD8+ cells and NK cells, serum levels of IL-2, IL-12 and sIL-2R in peripheral blood of patients with colorectal cancer; and to investigate the relationship between them. Methods Total 281 lymph nodes of 21 patients with colorectal cancer were collected. The positive expression of CK20 mRNA in lymph nodes was detect by reverse transcription-polymerase chain reaction (RT-PCR) and the metastasis in lymph nodes was detected by conventional pathological examination; the serum activities of CD4+ cells, CD8+ cells and NK cells were detected by flow cytometry and serum levels of IL-2, IL-12 and sIL-2R were detected by ELISA method in peripheral blood of patients with colorectal cancer. Results Among the positive metastasis in the 281 lymph nodes of the 21 patients, there were 16 (5.7%， 16/281) lymph nodes in 2 patients detected by pathological examination and 140 (49.8%， 140/281) lymph nodes in 10 patients by RT-PCR. There was a significant difference between the two measures in the aspects of the detection rate and the positive cases of lymph node metastasis in the 21 patients. Before operation, the serum activities of CD4+ cells, CD4+/CD8+ and NK cells, levels of IL-2 and IL-12 in 11 patients whose CK20 mRNA in regional lymph nodes were negative expression were higher than those in the other 8 patients whose lymph nodes metastasis were negative by conventional pathological examination but CK20 mRNA were positive expression （P＜0.05）; and the serum activity of CD8+ cells and level of sIL-2R in the former ones were lower than those in the latter ones （P＜0.05）. The serum activities of CD4+（r＝－0.769） cells, CD4+/CD8+（r=－0.755） and NK cells （r=－0.532）, the levels of IL-2 （r＝－0.834） and IL-12 （r＝－0.819） were negative correlated with the expression of CK20 mRNA （P＜0.05, P＜0.01）; and the activity of CD8+ cells （r=0.562） and level of sIL-2R （r=0.751） were positive correlated with the expression of CK20 mRNA （P＜0.05）. Conclusion The micrometastasis in lymph nodes is correlated significantly with the lower immune function of patients with colorectal cancer.
ObjectiveTo study the influence of psychological intervention on the immune function and psychological state in patients undergoing chemotherapy after radical operation of colorectal cancer. MethodSixty-four patients who underwent chemotherapy after eradicative resection of rectal cancer between August 2008 and August 2013 were randomly divided into control group and intervention group. Both the two groups of patients accepted conventional chemotherapy and nursing, while patients in the intervention group were also given psychological intervention. At the beginning of and 8 weeks after the therapy, self-evaluation of anxiety scale and depression self-rating scale were used to determine the psychological state (anxiety and depression) of patients in the two groups, and we evaluated the effect of psychological intervention. At the same time, the immune index and inflammatory cytokines were determined and compared between the two groups. ResultsBefore treatment, patients in both the two groups were accompanied by mild anxiety and depression. After psychological intervention, compared with the control group, anxiety and depression of patients in the intervention group were significantly alleviated (P<0.05). Before chemotherapy, patients in the two groups were not statistically different in the immune factor index (P>0.05). After chemotherapy, compared with the control group, natural killer cells, CD3+, CD4+, CD4+/CD8+, C-reactive protein, immunoglobulin (Ig) G, interleukin (IL)-10 level of the intervention group significantly increased (P<0.05), and IL-6 and tumor necrosis factor alpha expression decreased (P<0.05). CD8+, IgA and IgM were not significantly changed (P>0.05). ConclusionsPsychological intervention can alleviate anxiety and depression and improve the immune function in patients who undergo chemotherapy after radical operation of colorectal cancer, which is an effective auxiliary treatment.
ObjectiveTo explore the effects of pilose antler blood wine on bone mineral density and T-lymphocyte subsets of ovariectomized female Sprague Dawley (SD) rats. MethodsSixty female SD rats were randomly divided into six groups:normal control group, osteoporosis model group, pure ethylalcohol group, high dose of pilose antler blood wine group, medium dose of pilose antler blood wine group and low dose of pilose antler blood wine group. Normal control group and osteoporosis model group were given saline with the dose of 9.1 g/(kg·d). Pure ethylalcohol group was given pure ethylalcohol with the dose of 9.1 g/(kg·d). The doses of pilose antler blood wine were 4.5, 9.1 and 13.6 g/(kg·d) respectively in low, medium and high dose of pilose antler blood wine group. After the feeding of pilose antler blood wine for 30 days, the bone mineral density and T-lymphocyte subsets of rats were assessed. ResultsThe difference in bone mineral density among the three pilose antler blood wine groups was significant (P<0.05); the most obvious improvement of one mineral density was found in low and medium dose of pilose antler blood wine groups (P<0.05). The CD4+ T cells and CD4+/CD8+ significantly increased and CD8+ T cells proportion decreased significantly in pilose antler blood wine group compared with those in the osteoporosis model group (P<0.05). CD4+ T cells in medium dose group increased obviously compared with that in the high dose group (P<0.05). CD4+/CD8+ in low and medium dose groups obviously increased compared with that in high dose group (P<0.05). CD4+ T cells of medium dose group obviously increased compared with that in pure ethylalcohol group (P<0.05). CD8+ T cells in medium and low dose groups obviously decreased compared that in pure ethylalcohol group (P<0.05). CD4+/CD8+ in the three dose groups increased significantly compared with that in pure ethylalcohol group (P<0.05). ConclusionTree different doses of pilose antler blood wine could prevent the osteoporosis and improve the immune function. The effects of medium and low dose of groups were more obvious.
ObjectiveTo investigate effect of application of thymopentin during perioperative period on immune function of patients undergoing laparoscopic assisted radical resection for rectal carcinoma. MethodsForty-two patients undergoing laparoscopic assisted radical resection for rectal carcinoma from January 2015 to April 2015 in this hospital were collected. These patients were divided into study group and control group. The patients were received routine treatment in the control group. In addition to routine treatment same as the control group, the patients were received 2 mg thymopentin every day for a week in the study group. The changes of lymphocyte count, T lymphocyte subsets (CD3+, CD4+, CD8+, and CD4+/CD8+), and immunoglobulin (IgG, IgA, and IgM) were compared on the 1st day before surgery and on the 1st day and 5th day after surgery. ResultsThe lymphocyte count, T lymphocyte subsets, and immunoglobulin on the first day after surgery were significantly lower than those on the 1st day before surgery in these two groups (P < 0.05). The lymphocyte count, T lymphocyte subsets (except for CD4+/CD8+), and immunoglobulin (except for IgA) in the study group were significantly higher than those in the control group on the 5th day after surgery (P < 0.05). The changes of lymphocyte count, T lymphocyte subsets, and immunoglobulin in the study group had no significant differences between on the 5th day after surgery and on the 1st day before surgery (P > 0.05), but which on the 5th day afer surgery were significantly lower than those on the 1st day before surgery (P < 0.05) in the control group. ConclusionApplication of thymopentin during perioperative period could accelerate recovery of immune function after operation in patients undergoing laparoscopic assisted radical resection for rectal carcinoma.
ObjectiveTo evaluate clinical efficacy of mannatide for recurrent respiratory tract infection (RRTI) and its influence on immune function. MethodsThe Cochrane Library (Issue 12, 2013), PubMed, EMbase, CNKI, CBM, VIP and WanFang Data were searched for the randomized controlled trials (RCTs) that investigated the clinical and immune effect of mannatide in RRTI from inception to December 2013. Two reviewers independently screened studies according to the inclusion and exclusion criteria, extracted data and evaluated the methodological quality of included studies. Then meta-analysis was performed using the software RevMan 5.1.0. ResultsA total of 18 studies involving 1 481 patients were included. The results of meta-analysis showed that compared with the placebo group, the mannatide group was superior in total effectiveness and improving the levels of T-lymphocyte subsets and antibody (P < 0.05); compared with the levomisole group, the mannatide group was superior in total effectiveness and improving the level of T-lymphocyte subsets (P < 0.05), but not in improveming antibody level. ConclusionMannatide improves clinical efficacy in the treatment of RRTI and patients' immune function.